| 1 | Vulcan Materials Co | $7.8B | -- | -- |
| 2 | mueller service co. | $2.6B | 20,000 | 80 |
| 3 | bni top of tampa | $1.4B | 20,000 | 80 |
| 4 | elsevier's gold standard | $1.3B | 7,500 | 80 |
| 5 | tecta america west florida | $1.1B | 7,500 | 80 |
| 6 | mutual aid disaster relief | $817.0M | 20,000 | 70 |
| 7 | hcps leadership development | $396.4M | 20,000 | 70 |
| 8 | zap zimmerman advertising program @ usf | $343.3M | 3,000 | 70 |
| 9 | colliers-international | $196.5M | 3,000 | 70 |
| 10 | Coosemans Interproduce Divisions | $180.2M | 350 | 70 |
| 11 | diamante financial | $179.7M | 3,000 | 70 |
| 12 | paramount residential mortgage group - nmls no. 75243 | $179.7M | 3,000 | 70 |
| 13 | Dann Ocean Towing, Inc. | $171.1M | 125 | 70 |
| 14 | United Maritime Group | $171.1M | 125 | 70 |
| 15 | cafè barbera - the italian coffee house | $149.2M | 750 | 70 |
| 16 | Nosaj Online | $144.1M | 350 | 70 |
| 17 | Famous Tate Appliances & Bedding | $142.8M | 350 | 70 |
| 18 | Jerry Ulm Dodge, Inc. | $142.8M | 350 | 70 |
| 19 | Impact RTO Holdings | $142.8M | 350 | 70 |
| 20 | myMatrixx, by Evernorth | $128.6M | 350 | 70 |
| 21 | Nimble Health | $128.6M | 350 | 70 |
| 22 | Homeowners Choice Property & Casualty Insurance Company, Inc. | $128.6M | 350 | 70 |
| 23 | National Association of Benefits and Insurance Professionals Tampa Bay Chapter | $128.6M | 350 | 70 |
| 24 | Protector Plans | $128.6M | 350 | 70 |
| 25 | kindred hospital bay area tampa | $125.2M | 750 | 70 |
| 26 | pt star management inc | $121.9M | 3,000 | 70 |
| 27 | clean & park | $121.9M | 3,000 | 70 |
| 28 | Assured Group Services | $109.7M | 350 | 70 |
| 29 | HENDRY SHIPYARD JOINT VENTURE 1 | $96.0M | 350 | 60 |
| 30 | ReedTMS Logistics | $96.0M | 350 | 60 |
| 31 | HENDRY SHIPYARD VENTURE NO. 3, LLC | $96.0M | 350 | 60 |
| 32 | HENDRY SHIPYARD VENTURE NO. 4, LLC | $96.0M | 350 | 60 |
| 33 | tech trade hub | $95.8M | 125 | 60 |
| 34 | Beef 'O' Brady's | $91.5M | 720 | 60 |
| 35 | Gardner-Gibson, Inc. | $90.8M | 350 | 60 |
| 36 | Bloomin' Brands, Inc. | $88.2M | 694 | 60 |
| 37 | hassmann fasteners international | $78.9M | 350 | 60 |
| 38 | baldesigns de méxico | $77.3M | 350 | 60 |
| 39 | Allied Aerofoam Products, LLC | $76.6M | 350 | 60 |
| 40 | Magical Brands | $76.6M | 350 | 60 |
| 41 | Sun Metals Systems | $74.0M | 125 | 60 |
| 42 | American Metals Supply, Inc. | $74.0M | 125 | 60 |
| 43 | Resident Interface | $73.6M | 350 | 60 |
| 44 | Kay Bee Toys | $73.6M | 350 | 60 |
| 45 | MAH Advising LLC. | $73.6M | 350 | 60 |
| 46 | Benchmark International | $73.6M | 350 | 60 |
| 47 | Resident PreCollect | $73.6M | 350 | 60 |
| 48 | STS Lending® | $73.6M | 350 | 60 |
| 49 | Xpress Funding Group | $73.6M | 350 | 60 |
| 50 | Resident Advocate | $73.6M | 350 | 60 |
| 51 | quala sustainable container solutions | $71.8M | 750 | 60 |
| 52 | Kuhn Automotive Group | $69.4M | 350 | 60 |
| 53 | Reeves Import Motorcars | $69.4M | 350 | 60 |
| 54 | Center for Urban Transportation Research (CUTR) at the University of South Florida | $69.3M | 350 | 60 |
| 55 | Kisinger Campo & Associates, Corp. (KCA) | $69.3M | 350 | 60 |
| 56 | McGill Engineering, Inc. | $69.3M | 350 | 60 |
| 57 | ReconTRAC® | $66.6M | 350 | 60 |
| 58 | Computrol Inc | $66.5M | 350 | 60 |
| 59 | AES Data | $66.5M | 350 | 60 |
| 60 | xtratum.us | $64.4M | 125 | 60 |
| 61 | HSI TRADE COMPANY LLC | $64.4M | 125 | 60 |
| 62 | Alessi Bakeries | $63.5M | 125 | 60 |
| 63 | Harvest Singularity Inc | $63.5M | 125 | 60 |
| 64 | Grandbridge Senior Housing Investment Sales | $61.2M | 350 | 60 |
| 65 | FES Tampa Chapter | $60.9M | 350 | 60 |
| 66 | NextSphere Technologies | A Grid Dynamics Company | $59.6M | 350 | 60 |
| 67 | Wolters Kluwer TeamMate Audit Solutions | $59.6M | 350 | 60 |
| 68 | Route4Me | $59.6M | 350 | 60 |
| 69 | Verticent | $59.6M | 350 | 60 |
| 70 | Cync Software | $59.6M | 350 | 60 |
| 71 | NDS Systems, LC | $59.6M | 350 | 60 |
| 72 | RoboWorx, Inc. | $59.6M | 350 | 60 |
| 73 | Symbyo Technologies | $59.6M | 350 | 60 |
| 74 | HealthAxis Group | $59.6M | 350 | 60 |
| 75 | CAKE by Mad Mobile | $59.6M | 350 | 60 |
| 76 | Unknown.com | $59.6M | 350 | 60 |
| 77 | hire ed solutions (now part of ultimate medical academy) | $59.5M | 3,000 | 60 |
| 78 | tampa bay area writing project | $59.5M | 3,000 | 60 |
| 79 | future in med | $58.4M | 350 | 60 |
| 80 | harmony healthcare: a revenue cycle leadership and him staffing and solutions company | $58.4M | 350 | 60 |
| 81 | the rx care pharmacy | $58.4M | 350 | 60 |
| 82 | shp employment services, llc | $58.4M | 350 | 60 |
| 83 | dvbjj | $58.4M | 350 | 60 |
| 84 | Volet Hospitality | $57.5M | 350 | 60 |
| 85 | Evolution Parking and Guest Services | $57.5M | 350 | 60 |
| 86 | HI Hospitality Group | $57.5M | 350 | 60 |
| 87 | Burger 21 International and Grillsmith Restaurants | $57.5M | 350 | 60 |
| 88 | rcp america, inc | $57.2M | 350 | 60 |
| 89 | Vinik Sports Group | $56.7M | 350 | 60 |
| 90 | touch points solutions | $52.9M | 750 | 60 |
| 91 | health e systems dba healthesyst | $52.9M | 750 | 60 |
| 92 | ncompass is now tribridge | $52.9M | 750 | 60 |
| 93 | Shore Clean Solutions Tampa | $52.9M | 350 | 60 |
| 94 | Transcor Recycling LLC. | $52.9M | 350 | 60 |
| 95 | red vikings | $52.9M | 350 | 60 |
| 96 | D.R. Horton Tampa | $52.9M | 350 | 60 |
| 97 | Wal-Mark Contracting Group, LLC | $52.9M | 350 | 60 |
| 98 | Aph Stone Solutions | $52.9M | 350 | 60 |
| 99 | Construction Management Association of America Florida Chapter | $52.9M | 350 | 60 |
| 100 | PCS Civil, Inc. | $52.9M | 350 | 60 |