| 1 | Deutsche Bahn Aktiengesellschaft | €47.1B | 336,990 | 69 |
| 2 | Zalando SE | €10.4B | 14,194 | 69 |
| 3 | Delivery Hero SE | €9.9B | 47,981 | 69 |
| 4 | T.B. Asset Management GmbH | €5.5B | 922 | 69 |
| 5 | Charité - Universitätsmedizin Berlin | €2.3B | -- | 69 |
| 6 | Johanniter-Unfall-Hilfe e. V. | €2.3B | 33,517 | 69 |
| 7 | Rocket Internet SE | €2.2B | -- | 69 |
| 8 | amc international group | €1.4B | 7,500 | 80 |
| 9 | Deutsche Wohnen SE | €1.3B | -- | 69 |
| 10 | Strategy One GmbH | €1.1B | 2,003 | 69 |
| 11 | MEDIAN Unternehmensgruppe B.V. & Co. KG | €996.6M | 2,021 | 69 |
| 12 | MEDIAN Buchberg-Klinik GmbH & Co. KG | €996.6M | 2,021 | 69 |
| 13 | BMG RIGHTS MANAGEMENT GmbH | €545.0M | 834 | 69 |
| 14 | Bundesdruckerei | €526.4M | 2,088 | 65 |
| 15 | SEFE SECURING ENERGY FOR EUROPE GMBH | €489.9M | 1,563 | 70 |
| 16 | A.R.A.N.A. Verwaltungs GmbH | €459.0M | -- | 69 |
| 17 | Gewobag | €427.7M | 658 | 65 |
| 18 | Atotech Deutschland GmbH & Co. KG | €412.1M | 335 | 69 |
| 19 | Caritasverband für das Erzbistum Berlin e. V. | €412.0M | 5,407 | 69 |
| 20 | Stiftung Preußischer Kulturbesitz | €400.9M | 2,099 | 69 |
| 21 | Onyx Germany GmbH | €400.4M | 13 | 69 |
| 22 | bew berliner energie und wärme ag | €384.8M | 3,000 | 70 |
| 23 | Toll Collect GmbH | €356.1M | 622 | 69 |
| 24 | Johanniter Seniorenhäuser GmbH | €324.2M | 5,493 | 69 |
| 25 | FRÖBEL e.V. | €287.5M | 4,905 | 69 |
| 26 | Stephanus-Stiftung | €256.5M | 3,662 | 69 |
| 27 | SHF Communication Technologies AG | €250.3M | 148 | 69 |
| 28 | Evangelisches Jugend- und Fürsorgewerk e.V. | €235.7M | 3,686 | 69 |
| 29 | Aaziko Germany | €229.6M | 350 | 70 |
| 30 | Konrad-Adenauer-Stiftung e.V. | €223.7M | 560 | 69 |
| 31 | Mister Spex SE | €217.0M | 1,293 | 69 |
| 32 | EJF gemeinnützige AG | €213.8M | 4,284 | 69 |
| 33 | dastn gmbh | resources relocation & offshoring | custom software development | €211.5M | 3,000 | 70 |
| 34 | Deutsches Rotes Kreuz e. V. | €210.7M | 688 | 69 |
| 35 | Aristo Pharma GmbH | €208.1M | -- | 69 |
| 36 | JORDAHL | €207.2M | 350 | 70 |
| 37 | GE GRID GMBH | €204.6M | 2,021 | 69 |
| 38 | Iberdrola Energie Deutschland GmbH | €200.8M | -- | 69 |
| 39 | BERLIN LIVING 1 GmbH | €199.0M | 4,348 | 69 |
| 40 | Lautsprecher Teufel GmbH | €197.7M | 319 | 69 |
| 41 | Exclusive Networks Deutschland GmbH | €190.6M | 80 | 69 |
| 42 | young freemasons | €190.0M | 20,000 | 70 |
| 43 | VAMED Health Project GmbH | €185.1M | 93 | 69 |
| 44 | plilend | €179.7M | 3,000 | 70 |
| 45 | accenture global investments india private limited | €179.7M | 3,000 | 70 |
| 46 | ifp Institut für Produktqualität | €177.9M | 350 | 70 |
| 47 | Estudio Juridico | €176.4M | 1,753 | 70 |
| 48 | Cabka Group GmbH | €170.6M | 655 | 69 |
| 49 | airberlin technik GmbH | €160.1M | 14,403 | 69 |
| 50 | ]init[ Aktiengesellschaft für digitale Kommunikation | €152.6M | -- | 69 |
| 51 | Haus Tec GmbH | €144.1M | 350 | 70 |
| 52 | Hertha BSC GmbH & Co. KGaA | €139.3M | 2 | 69 |
| 53 | WWF Deutschland | €128.1M | 502 | 69 |
| 54 | Sportstech Brands Holding GmbH | €123.8M | 347 | 69 |
| 55 | DPF Group | €123.6M | 350 | 70 |
| 56 | N26 Bank SE | €120.4M | 700 | 69 |
| 57 | Brunner Berlin Beteiligungen GmbH | €118.8M | 381 | 69 |
| 58 | COSMO CONSULT AG | €117.6M | -- | 69 |
| 59 | Stiftung Deutsche Klassenlotterie Berlin | €115.4M | -- | 69 |
| 60 | Taiyol Lubricants GmbH | Asia's No. 1 Brand | €109.7M | 350 | 70 |
| 61 | Arbeiterwohlfahrt Bundesverband e.V. | €109.3M | -- | 69 |
| 62 | GRTgaz Deutschland GmbH | €106.6M | 19 | 69 |
| 63 | Lebenshilfe Berlin e.V. | €105.9M | 2,241 | 69 |
| 64 | Schrobsdorff Holding AG | €105.7M | -- | 69 |
| 65 | Schrobsdorff Bau Aktiengesellschaft | €105.7M | -- | 69 |
| 66 | Victor's Bau + Wert AG | €105.3M | 3 | 69 |
| 67 | Verve Group Europe GmbH | €103.1M | 71 | 69 |
| 68 | Bundesstiftung Magnus Hirschfeld | €99.7M | 7 | 69 |
| 69 | PartsTrade 24 | €96.0M | 350 | 60 |
| 70 | FCG Germany (Formerly Pohl Consulting & Associates) | €95.8M | 125 | 60 |
| 71 | The Trade Council of Denmark in Germany | €95.8M | 125 | 60 |
| 72 | RCS Global Group | €95.8M | 125 | 60 |
| 73 | audibene GmbH | €93.4M | 76 | 69 |
| 74 | Pump Technology Solutions PS GmbH | €90.2M | 2,021 | 69 |
| 75 | SumUp Services GmbH | €88.7M | -- | 69 |
| 76 | Peppermint Holding GmbH | €87.6M | 2,021 | 69 |
| 77 | HomeToGo GmbH | €86.0M | 2,020 | 69 |
| 78 | Storengy Deutschland GmbH | €83.4M | 55 | 69 |
| 79 | GetYourGuide Deutschland GmbH | €83.4M | 67 | 69 |
| 80 | B&O Bau und Gebäudetechnik GmbH & Co. KG | €83.2M | 100 | 69 |
| 81 | KORSCH AG | €81.9M | 350 | 60 |
| 82 | S-Servicepartner Berlin GmbH | €81.6M | 150 | 69 |
| 83 | ADTRAN GmbH | €80.9M | -- | 69 |
| 84 | Rosa-Luxemburg-Stiftung - Gesellschaftsanalyse und Politische Bildung e. V. | €79.5M | 275 | 69 |
| 85 | Off-Grid Water Alliance | €79.1M | 350 | 60 |
| 86 | FLEXIM Flexible Industriemeßtechnik GmbH | €75.6M | 447 | 69 |
| 87 | PREVENT DEV GmbH Design Entwicklung Vertrieb | €75.2M | 2 | 69 |
| 88 | Esteve Pharmaceuticals GmbH | €74.3M | 86 | 69 |
| 89 | Krypto News Deutschland | €73.6M | 350 | 60 |
| 90 | Moss | Spend smarter | €73.6M | 350 | 60 |
| 91 | DeineRate | €73.6M | 350 | 60 |
| 92 | MAEL GROUPS FINANCE | €73.6M | 350 | 60 |
| 93 | IVU Traffic Technologies AG | €71.1M | 1,000 | 69 |
| 94 | CCG-Commercial Coordination Germany GmbH | €71.1M | 578 | 69 |
| 95 | Advanced Technical Solutions Dubai | €70.0M | 350 | 60 |
| 96 | Save the Children Deutschland e.V. | €70.0M | 276 | 69 |
| 97 | Ableton AG | €69.9M | 15 | 69 |
| 98 | Matsmart - Motatos | €69.6M | 350 | 60 |
| 99 | Großkundenzentrum Berlin-Brandenburg | €69.4M | 350 | 60 |
| 100 | remerge GmbH | €66.8M | 2,021 | 69 |