| 1 | Paychex Holdings, LLC | $5.6B | -- | 75 |
| 2 | prime roofers rochester | $1.1B | 7,500 | 80 |
| 3 | MarketWise, Inc. | $512.4M | -- | 80 |
| 4 | TRANSCAT INC | $278.4M | -- | 75 |
| 5 | home builders & remodelers association of illinois | $196.5M | 3,000 | 70 |
| 6 | university of rochester - department of history | $186.6M | 20,000 | 70 |
| 7 | university of rochester – school of medicine and dentistry | $186.6M | 20,000 | 70 |
| 8 | CONSTELLATION BRANDS U.S. OPERATIONS, INC. | $177.9M | 350 | 70 |
| 9 | Palmer Food Services | $142.8M | 350 | 70 |
| 10 | APICS Rochester | $142.8M | 350 | 70 |
| 11 | high falls operating company, llc | $142.3M | 350 | 70 |
| 12 | Reichel Foods Inc. | $142.3M | 350 | 70 |
| 13 | DiMarco Constructors | $137.1M | 350 | 70 |
| 14 | depaul community services dental insurance plan | $125.2M | 750 | 70 |
| 15 | jsc management group | $122.7M | 3,000 | 70 |
| 16 | the pike companies, ltd. | $113.3M | 750 | 70 |
| 17 | crenlo | $91.8M | 750 | 60 |
| 18 | Quality Vision International (QVI®) | $81.9M | 350 | 60 |
| 19 | OGP – Optical Gaging Products | $81.9M | 350 | 60 |
| 20 | Val Tech Holdings, Inc. | $80.1M | 350 | 60 |
| 21 | Innatech | $80.1M | 350 | 60 |
| 22 | rochling advent tool & mold lp | $78.9M | 350 | 60 |
| 23 | First Federal Savings Bank, FFSB | $73.6M | 350 | 60 |
| 24 | Chase Investment Service | $73.6M | 350 | 60 |
| 25 | the doan family of dealerships | $69.7M | 750 | 60 |
| 26 | Frontier Food Brokerage (now Epic Sales Partners) | $69.6M | 350 | 60 |
| 27 | Arr Aye Tea | $69.6M | 350 | 60 |
| 28 | Revolution Concrete Mixers | $69.4M | 350 | 60 |
| 29 | Hyzon Motors | $69.4M | 350 | 60 |
| 30 | MONROE MOTOR PRODUCTS CORP. | $69.4M | 350 | 60 |
| 31 | ASCE Rochester Section | $69.3M | 350 | 60 |
| 32 | Ziebart | The Mattiacio Group | $66.6M | 350 | 60 |
| 33 | Bob Johnson Auto Group | $66.6M | 350 | 60 |
| 34 | SYNTEC TECHNOLOGIES, INC. DBA SY | $66.5M | 350 | 60 |
| 35 | REVELATION, INC. | $64.6M | 350 | 60 |
| 36 | can-am consultants, inc | $64.3M | 750 | 60 |
| 37 | Village Bakery & Cafe - Armory | $63.5M | 125 | 60 |
| 38 | NYS Food Processors | $63.5M | 125 | 60 |
| 39 | Netsmartz - Custom Mobile Applications | $59.6M | 350 | 60 |
| 40 | Trbhi Inc | $59.6M | 350 | 60 |
| 41 | starbridge services inc | $58.4M | 350 | 60 |
| 42 | freedom therapy, ot, pt, slp, pllc | $58.4M | 350 | 60 |
| 43 | united states of america at carbusa llc | $57.2M | 350 | 60 |
| 44 | ADMAR Construction Equipment and Supplies | $52.9M | 350 | 60 |
| 45 | Elmer W. Davis, Inc. - Commercial Roofing | $52.9M | 350 | 60 |
| 46 | BELL CONSTRUCTORS, LLC (BELL CONSTRUCTORS, LLC) | $52.9M | 350 | 60 |
| 47 | mark iv construction company | $52.9M | 350 | 60 |
| 48 | Billitier Electric, Inc | $52.9M | 350 | 60 |
| 49 | Vaccinex | $52.2M | 125 | 60 |
| 50 | f. m. office express, inc. | $51.5M | 125 | 60 |
| 51 | Abrasive-Tool Corp. | $51.5M | 125 | 60 |
| 52 | O'CONNOR CHEVROLET, INC | $51.0M | 125 | 60 |
| 53 | Big City Tees | $51.0M | 125 | 60 |
| 54 | ARROW ACE HARDWARE, INC. | $51.0M | 125 | 60 |
| 55 | INTERNATIONAL ASSOCIATION OF HEAT & FROST INSULATORS ASBESTOS WORKERS | $45.9M | 125 | 60 |
| 56 | The MedAmerica Insurance Companies | $45.9M | 125 | 60 |
| 57 | Globe Life National Income Division: Gryska Organization | $45.9M | 125 | 60 |
| 58 | Light Fabrications/Fabrico | $45.0M | 125 | 60 |
| 59 | jubilee capital funding® | $44.9M | 750 | 60 |
| 60 | paperhelp.org | $43.9M | 350 | 60 |
| 61 | erdman, anthony holding company, inc | $42.9M | 350 | 60 |
| 62 | Piper Team | $40.3M | 350 | 60 |
| 63 | University of Rochester - Biomedical Engineering (BME) | $40.1M | 350 | 60 |
| 64 | computer science undergraduate council | $40.1M | 350 | 60 |
| 65 | Saunders College of Business at RIT | $40.1M | 350 | 60 |
| 66 | RCTC Center for Business and Workforce Education @RCTCBWE | $40.1M | 350 | 60 |
| 67 | st. john fisher college | $40.1M | 350 | 60 |
| 68 | College of Art and Design at RIT | $40.1M | 350 | 60 |
| 69 | CJWinter - Cold Root Rolling | $39.2M | 125 | 60 |
| 70 | AEOP Apprenticeships & Fellowships | $36.4M | 350 | 60 |
| 71 | The DePrez Group of Travel Companies | $35.5M | 125 | 60 |
| 72 | Monroe County Caseworkers | $35.2M | 350 | 60 |
| 73 | City of Rochester, New Hampshire | $35.2M | 350 | 60 |
| 74 | MONROE COUNTY AIRPORT AUTHORITY | $34.3M | 125 | 60 |
| 75 | SILVAROLE TRUCKING INC | $34.3M | 125 | 60 |
| 76 | King Carriers Inc | $34.3M | 125 | 60 |
| 77 | depaul community services inc | $33.5M | 350 | 60 |
| 78 | Sturdell Industries Inc | $33.3M | 125 | 60 |
| 79 | Jasco Tools, Inc. | $33.3M | 125 | 60 |
| 80 | K.W. THOMPSON TOOL COMPANY, INC | $33.3M | 125 | 60 |
| 81 | Taxi Mobile Solutions | $33.1M | 350 | 60 |
| 82 | AISmartz | $33.1M | 350 | 60 |
| 83 | CloudCheckr (part of Spot by NetApp) | $33.1M | 350 | 60 |
| 84 | renewal by andersen of western new york | $32.4M | 125 | 60 |
| 85 | rochester concrete products, inc. | $32.4M | 125 | 60 |
| 86 | Prosoft IT Services Inc | $31.1M | 350 | 60 |
| 87 | Schott Distributing Co Inc | $30.8M | 125 | 60 |
| 88 | Brothers International Food Holdings, LLC | $30.8M | 125 | 60 |
| 89 | Hybridge Dental | $30.6M | 125 | 60 |
| 90 | RLab.Independent | $30.6M | 125 | 60 |
| 91 | Express Employment Professionals Rochester New York | $30.0M | 350 | 60 |
| 92 | TES Staffing Inc. | $30.0M | 350 | 60 |
| 93 | ShiftHop, LLC. | $30.0M | 350 | 60 |
| 94 | リクルートr&dスタッフィング | $30.0M | 350 | 60 |
| 95 | Davenport Machine, Inc. | $29.2M | 125 | 60 |
| 96 | Marley Cooling Tower | $29.2M | 125 | 60 |
| 97 | QED Technologies International LLC | $29.2M | 125 | 60 |
| 98 | Bowman Tool & Machining, Inc. | $29.2M | 125 | 60 |
| 99 | PJ Industrial Supply | $29.2M | 125 | 60 |
| 100 | Spex Precision Machine Technologies Inc. | $29.2M | 125 | 60 |