| 1 | hertz dealer direct jacksonville | $3.8B | 20,000 | 80 |
| 2 | us navy medicine programs | $3.3B | 20,000 | 80 |
| 3 | ess duval county | $2.3B | 20,000 | 80 |
| 4 | bi-lo holding, llc | $1.5B | 7,500 | 80 |
| 5 | HCI Group, Inc. | $900.9M | -- | 85 |
| 6 | asce jacksonville branch | $594.1M | 3,000 | 70 |
| 7 | the clear choice! team | $491.4M | 7,500 | 70 |
| 8 | coggin graduate and executive programs | $343.3M | 3,000 | 70 |
| 9 | bda+rp | $278.9M | 3,000 | 70 |
| 10 | Ascension St. Vincent's | $199.9M | 1,198 | 70 |
| 11 | black diamond performance reporting, llc | $179.7M | 3,000 | 70 |
| 12 | Beaver Street Fisheries | $177.9M | 350 | 70 |
| 13 | MARINE TRANSPORT MANAGEMENT, INC. | $171.1M | 125 | 70 |
| 14 | Crowley Petroleum Services, Inc. | $171.1M | 125 | 70 |
| 15 | TOTE Group | $171.1M | 125 | 70 |
| 16 | HANANIA AUTOMOTIVE MANAGEMENT CORP | $142.8M | 350 | 70 |
| 17 | Rail Rate Advisors | $142.8M | 350 | 70 |
| 18 | Champion Brands, Inc | $142.8M | 350 | 70 |
| 19 | hanania automotive | $142.6M | 750 | 70 |
| 20 | foodonics international, inc. | $142.3M | 350 | 70 |
| 21 | iatse local 115 | $139.3M | 750 | 70 |
| 22 | Rogers Benefit Group - Jacksonville, FL | $128.6M | 350 | 70 |
| 23 | Nimnicht Insurance Services , LLC | $128.6M | 350 | 70 |
| 24 | Global Claims Services | $128.6M | 350 | 70 |
| 25 | ua local 234 jacksonville | $113.3M | 750 | 70 |
| 26 | ibew local 508 & east div ga chap. neca pension | $113.3M | 750 | 70 |
| 27 | PHB Transport | $96.0M | 350 | 60 |
| 28 | Interrail Inc. | $96.0M | 350 | 60 |
| 29 | Jacksonville Port Authority (JAXPORT) | $95.8M | 125 | 60 |
| 30 | Tremron Group | $90.8M | 350 | 60 |
| 31 | US Army Aviation | $79.1M | 350 | 60 |
| 32 | Marble Today, Inc | $74.0M | 125 | 60 |
| 33 | Enduris Inc | $74.0M | 125 | 60 |
| 34 | Builder's Best, Inc. | $74.0M | 125 | 60 |
| 35 | Whit Davis Lumber Company | $74.0M | 125 | 60 |
| 36 | Dual Action Windows, LLC | $74.0M | 125 | 60 |
| 37 | Cain & Bultman, Inc | $74.0M | 125 | 60 |
| 38 | ThermaFoam Arkanasas | $74.0M | 125 | 60 |
| 39 | Florida Metal Products, Inc. - Flamco | $74.0M | 125 | 60 |
| 40 | Arias Agencies Jax | $73.6M | 350 | 60 |
| 41 | Credit Game University | $73.6M | 350 | 60 |
| 42 | Intuition College Savings Solutions | $73.6M | 350 | 60 |
| 43 | FAB&T | $73.6M | 350 | 60 |
| 44 | proficient auto logistics | $71.8M | 750 | 60 |
| 45 | auto logistics | $71.8M | 750 | 60 |
| 46 | florida east coast railway corp f/n/a fecr rail corp | $71.8M | 750 | 60 |
| 47 | ASHE - Northeast Florida | $70.2M | 350 | 60 |
| 48 | sysco jacksonville | $69.6M | 350 | 60 |
| 49 | Stevenson Automotive Group | $69.4M | 350 | 60 |
| 50 | O'Steen Automotive Group, Inc. | $69.4M | 350 | 60 |
| 51 | RV Care Pros | $69.4M | 350 | 60 |
| 52 | BRUMOS MOTOR CARS INC | $66.6M | 350 | 60 |
| 53 | brumos automotive | $66.6M | 350 | 60 |
| 54 | Shaw's Southern Belle Frozen Foods, Inc. | $63.5M | 125 | 60 |
| 55 | Network FOB | $62.9M | 350 | 60 |
| 56 | Prism IT Corp. | $59.6M | 350 | 60 |
| 57 | NORTH FLORIDA SALES TRIPLE P DISTRIBUTING CO., INC | $59.6M | 350 | 60 |
| 58 | Radius Solutions, LLC | $59.6M | 350 | 60 |
| 59 | taylor, fannie e home for the aged care center inc | $58.4M | 350 | 60 |
| 60 | HCA Florida Memorial Hospital | $56.4M | 338 | 60 |
| 61 | J. E. Abercrombie, Inc. | $52.9M | 350 | 60 |
| 62 | ibew local 508 east div ga chapt neca h&w fund | $52.9M | 350 | 60 |
| 63 | Regency Electric Company, Inc. | $52.9M | 350 | 60 |
| 64 | DAVIS MAIL SERVICES, INC | $52.9M | 350 | 60 |
| 65 | BeLit, Inc. | $52.9M | 350 | 60 |
| 66 | Sunshine State Plumbing | $52.9M | 350 | 60 |
| 67 | misener marine/orion marine construction | $52.9M | 350 | 60 |
| 68 | Summit Contracting Group | We're Hiring! | $52.9M | 350 | 60 |
| 69 | Monarch Homes | $52.2M | 125 | 60 |
| 70 | bestbet poker, simulcast and racing | $52.0M | 750 | 60 |
| 71 | What Chefs Want Jacksonville | $51.5M | 125 | 60 |
| 72 | Flowergeneral.com by Carlstedt's | $51.5M | 125 | 60 |
| 73 | Mercury Luggage Manufacturing Company | $51.5M | 125 | 60 |
| 74 | Tsumura International | $51.0M | 125 | 60 |
| 75 | Moore Buick Pontiac GMC Truck, Inc. | $51.0M | 125 | 60 |
| 76 | Coggin Buick-GMC of Orange Park | $51.0M | 125 | 60 |
| 77 | Coggin Chevrolet LLC | $51.0M | 125 | 60 |
| 78 | Hans-Mill Corp | $51.0M | 125 | 60 |
| 79 | Setzer's & Company Inc | $51.0M | 125 | 60 |
| 80 | Mike Shad Ford, Inc. | $51.0M | 125 | 60 |
| 81 | SimplyStamps | $51.0M | 125 | 60 |
| 82 | AllStuff420 | $51.0M | 125 | 60 |
| 83 | StarFleetX | $51.0M | 125 | 60 |
| 84 | T&L Council - Jacksonville Chamber of Commerce | $51.0M | 125 | 60 |
| 85 | Reliance Supply, Inc., a BlackHawk Industrial Company | $51.0M | 125 | 60 |
| 86 | certified steak and seafood | $50.8M | 125 | 60 |
| 87 | EAST CAROLINA MECHANICAL LLC | $49.0M | 125 | 60 |
| 88 | QUICK TIE PRODUCTS, INC. | $49.0M | 125 | 60 |
| 89 | natural specialty sales | $47.5M | 350 | 60 |
| 90 | itel | $45.9M | 125 | 60 |
| 91 | Omega Home & Auto Care | $45.9M | 125 | 60 |
| 92 | LotSolutions | $45.9M | 125 | 60 |
| 93 | Carolina Casualty (a Berkley Company) | $45.9M | 125 | 60 |
| 94 | NABIP Jacksonville | $45.9M | 125 | 60 |
| 95 | Triserv Alliance LLC | $45.9M | 125 | 60 |
| 96 | Life of the South | $45.9M | 125 | 60 |
| 97 | GuideWell Insurance Agency | $45.9M | 125 | 60 |
| 98 | Medcom Benefit Solutions | $45.9M | 125 | 60 |
| 99 | Greene-Hazel Insurance Group | $45.9M | 125 | 60 |
| 100 | US Assure | $45.9M | 125 | 60 |