| 1 | widgetcorp | $2.6B | 20,000 | 80 |
| 2 | university of florida laboratory for systems medicine | $2.3B | 20,000 | 80 |
| 3 | SELECT WATER SOLUTIONS, LLC | $1.5B | -- | 75 |
| 4 | avison young│north florida | $491.4M | 7,500 | 70 |
| 5 | northeast georgia medical center graduate medical education | $251.2M | 20,000 | 70 |
| 6 | almark holdings, inc | $142.3M | 350 | 70 |
| 7 | IFT Florida | $142.3M | 350 | 70 |
| 8 | Agora, Inc. | $141.1M | -- | 85 |
| 9 | Watermelon Insurance Group | $128.6M | 350 | 70 |
| 10 | Tower Hill Insurance Group | $128.6M | 350 | 70 |
| 11 | Nsure Network | $128.6M | 350 | 70 |
| 12 | Florida Farm Bureau Insurance - Agents | $128.6M | 350 | 70 |
| 13 | King Insurance Partners | $128.6M | 350 | 70 |
| 14 | mckibbon brothers, inc. | $121.9M | 3,000 | 70 |
| 15 | NORA, An Association of Responsible Recyclers | $109.7M | 350 | 70 |
| 16 | RESOURCE TRANSPORT, LLC | $96.0M | 350 | 60 |
| 17 | BSBC Zaxbys | $86.3M | 350 | 60 |
| 18 | medi-gators program | $85.8M | 750 | 60 |
| 19 | Pittman Dental Laboratory and Surgical Solutions | $85.6M | 350 | 60 |
| 20 | llama's leetcode @ ten am | $84.5M | 3,000 | 60 |
| 21 | Geveko Markings North America | $74.0M | 125 | 60 |
| 22 | John Hancock Funds Inc | $73.6M | 350 | 60 |
| 23 | ims gear georgia inc. | $66.6M | 350 | 60 |
| 24 | CMS Mechanical Solutions | $63.5M | 125 | 60 |
| 25 | Syfan Logistics | $62.9M | 350 | 60 |
| 26 | georgia mountain community services | $58.4M | 350 | 60 |
| 27 | Hilton University of Florida | $57.5M | 350 | 60 |
| 28 | S.W. Rodgers Co. Inc. | $52.9M | 350 | 60 |
| 29 | Superior Paving Corp. | $52.9M | 350 | 60 |
| 30 | a's pro plumbing gainesville fl experts | $51.5M | 125 | 60 |
| 31 | Premier PPE Supply | $51.0M | 125 | 60 |
| 32 | Mugele of America, Inc. | $51.0M | 125 | 60 |
| 33 | Arrow Lines Transport LLC | $51.0M | 125 | 60 |
| 34 | san felasco nurseries, inc. | $50.8M | 125 | 60 |
| 35 | realprotect | $45.9M | 125 | 60 |
| 36 | Insurance Intermediaries | $45.9M | 125 | 60 |
| 37 | Florida Farm Bureau Casualty Insurance Company | $45.9M | 125 | 60 |
| 38 | City of Gainesville Public Utilities | $44.9M | 350 | 60 |
| 39 | ATM, a Geosyntec Company | $41.1M | 30 | 60 |
| 40 | Underwater Services Intl | $41.1M | 30 | 60 |
| 41 | WUFT News | $40.9M | 350 | 60 |
| 42 | SSTP STUDENTS AND ALUMNI | $40.1M | 350 | 60 |
| 43 | UF Business Undergraduate Mentorship Program | $40.1M | 350 | 60 |
| 44 | UF Herbert Wertheim College of Engineering | $40.1M | 350 | 60 |
| 45 | uf inter-residence hall association | $40.1M | 350 | 60 |
| 46 | University of Florida Online | $40.1M | 350 | 60 |
| 47 | McKnight Brain Institute of the University of Florida | $40.1M | 350 | 60 |
| 48 | University of Florida Department of Agricultural and Biological Engineering | $40.1M | 350 | 60 |
| 49 | UF Department of Chemical Engineering | $40.1M | 350 | 60 |
| 50 | University of Florida College of Public Health and Health Professions | $40.1M | 350 | 60 |
| 51 | UF Genetics & Genomics Graduate Program | $40.1M | 350 | 60 |
| 52 | UF/IFAS Environmental Horticulture Department | $40.1M | 350 | 60 |
| 53 | iot students club | $40.1M | 350 | 60 |
| 54 | Hispanic Student Association at UF | $40.1M | 350 | 60 |
| 55 | college of medecine | $39.2M | 125 | 60 |
| 56 | Oilfield Equipment Sales, LLC | $39.2M | 125 | 60 |
| 57 | EquusNox | $39.2M | 125 | 60 |
| 58 | Site Safe Solutions | $39.2M | 125 | 60 |
| 59 | regius investigations & protective services | $38.7M | 750 | 60 |
| 60 | uf college of medicine finance & administration fellowship and internship program | $37.7M | 3,000 | 60 |
| 61 | Florida Institute for Cybersecurity Research | $36.4M | 350 | 60 |
| 62 | IFS Powder Coatings, Inc. | $36.2M | 125 | 60 |
| 63 | EGX Chemical | $36.2M | 125 | 60 |
| 64 | City of Gainesville, Texas | $35.2M | 350 | 60 |
| 65 | Gator Party | $35.2M | 350 | 60 |
| 66 | Dress Up Corporation | $34.6M | 350 | 60 |
| 67 | TURBO TRUCK CENTER INC | $34.3M | 125 | 60 |
| 68 | PARS Fleet Vehicle Relocation and Transport | $34.3M | 125 | 60 |
| 69 | Gatorloop | $34.3M | 125 | 60 |
| 70 | GTO 2000, Inc | $34.3M | 125 | 60 |
| 71 | Florida Food Service | $30.8M | 125 | 60 |
| 72 | mcdonalds of northeast georgia | $30.7M | 750 | 60 |
| 73 | CHANDLER EQUIPMENT COMPANY, INC | $29.2M | 125 | 60 |
| 74 | Cantrell•Gainco Group, Inc. | $29.2M | 125 | 60 |
| 75 | Advanced Pedestals, Ltd | $28.6M | 125 | 60 |
| 76 | PETROFLEX N.A. LTD | $28.6M | 125 | 60 |
| 77 | Petroflex N.A., Ltd | $28.6M | 125 | 60 |
| 78 | Lanier Color Company | $28.6M | 125 | 60 |
| 79 | Climate Action Gator | $28.2M | 125 | 60 |
| 80 | lasercraft technologies, inc. | $28.2M | 125 | 60 |
| 81 | niverplast usa | $28.2M | 125 | 60 |
| 82 | MARTIN DOCKS, INC. | $27.4M | 125 | 60 |
| 83 | Louver Shop | $27.4M | 125 | 60 |
| 84 | Merchant Cash Group | $26.3M | 125 | 60 |
| 85 | Maverick Residential Mortgage | $26.3M | 125 | 60 |
| 86 | Foothills Community Bank | $26.3M | 125 | 60 |
| 87 | Environmental Consulting & Technology, Inc. (ECT) | $26.1M | 350 | 60 |
| 88 | Takenaka USA Corp | $25.1M | 125 | 60 |
| 89 | CAUSSEAUX, HEWETT & WALPOLE, INC | $25.0M | 125 | 60 |
| 90 | Duval Motorcars | $24.8M | 125 | 60 |
| 91 | Wade Raulerson Pontiac, Buick, GMC | $24.8M | 125 | 60 |
| 92 | Jones Edmunds | $24.8M | 125 | 60 |
| 93 | American Society of Civil Engineers, University of Florida | $24.8M | 125 | 60 |
| 94 | the professional care management & advocacy group | $24.7M | 350 | 60 |
| 95 | Solar Gators | $23.8M | 125 | 60 |
| 96 | Eta Kappa Nu (HKN) Epsilon Sigma | $23.7M | 125 | 60 |
| 97 | Brazilian Cultural Arts Exchange, Gainesville FL | $23.2M | 125 | 60 |
| 98 | Fightin' Gator Marching Band | $23.1M | 350 | 60 |
| 99 | polish american chamber of commerce of eastern usa | $23.0M | 30 | 60 |
| 100 | Keller Williams Realty Lanier Partners | $22.9M | 350 | 60 |