| 1 | W Z WISCO INC | $57.5M | 350 | 60 |
| 2 | Commonwealth Construction Corp. | $49.0M | 125 | 60 |
| 3 | diversified infrastructure services, inc. (dis) | $44.9M | 350 | 60 |
| 4 | Marian University of Fond du Lac | $40.1M | 350 | 60 |
| 5 | City of Fond du Lac | $35.2M | 350 | 60 |
| 6 | Marchant Schmidt | $29.2M | 125 | 60 |
| 7 | marchant schmidt inc. - vms group | $28.2M | 125 | 60 |
| 8 | Hometown Bank (Fond Du Lac, WI) | $26.3M | 125 | 60 |
| 9 | WiLL | Wisconsin Lighting Lab® | $23.7M | 125 | 60 |
| 10 | Home Box Office | $21.8M | 350 | 60 |
| 11 | LENZ SALES & SERVICE, INC | $21.3M | 125 | 60 |
| 12 | taylored rehab, llc | $20.9M | 125 | 60 |
| 13 | fond du lac cty acute psych unit | $20.9M | 125 | 60 |
| 14 | Fond Du Lac Clinic | $19.2M | 350 | 60 |
| 15 | equix energy | $18.9M | 125 | 60 |
| 16 | Natural Stone Veneers Inc | $17.8M | 30 | 60 |
| 17 | University of Wisconsin-Fond du Lac | $14.3M | 125 | 60 |
| 18 | Parking Equipment Covers | $13.0M | 125 | 60 |
| 19 | City of Fond du Lac Police Dept | $12.6M | 125 | 60 |
| 20 | Police Departmen | $12.6M | 125 | 60 |
| 21 | OnlineBootStore / BootBiz | $12.2M | 30 | 60 |
| 22 | Osborn & Son Trucking | $12.2M | 30 | 60 |
| 23 | Crust N Crumb Cafe | $12.2M | 30 | 60 |
| 24 | Kara Foods | $12.2M | 30 | 60 |
| 25 | o'connor oil corporation | $11.6M | 125 | 60 |
| 26 | mike shannon automotive, inc. | $11.6M | 125 | 60 |
| 27 | Jackson Kahl Insurance Services | $11.0M | 30 | 60 |
| 28 | Guaranty Closing and Title Services, Inc. | $11.0M | 30 | 60 |
| 29 | Guaranty Services Group Inc | $11.0M | 30 | 60 |
| 30 | Hierl Insurance, Inc. | $11.0M | 30 | 60 |
| 31 | Alliance Manufacturing, Inc. | $10.8M | 30 | 60 |
| 32 | Merwin Oil Co | $9.4M | 30 | 50 |
| 33 | Whealon Towing & Service Inc. | $8.2M | 30 | 50 |
| 34 | völker | $8.2M | 125 | 50 |
| 35 | ServiceMaster Clean Residential & Commercial Services | $7.8M | 125 | 50 |
| 36 | AMC of Wisconsin | $7.8M | 30 | 50 |
| 37 | Manowske Welding Corp | $7.0M | 30 | 50 |
| 38 | Integrity Saw & Tool Inc | $7.0M | 30 | 50 |
| 39 | Tobin Machining Inc | $7.0M | 30 | 50 |
| 40 | Creative Structural Solutions | $7.0M | 30 | 50 |
| 41 | Nimmer Marine Svc | $6.8M | 5 | 50 |
| 42 | Guide-on Step, LLC | $6.8M | 5 | 50 |
| 43 | Lutheran Homes & Health Services | $6.7M | 125 | 50 |
| 44 | Lutheran Homes & Health Services, Inc. | $6.7M | 125 | 50 |
| 45 | Quality Packaging Systems, Inc. | $6.6M | 30 | 50 |
| 46 | fond du lac ymca | $6.5M | 350 | 50 |
| 47 | excel technologies | $6.5M | 125 | 50 |
| 48 | Cm Express | $6.3M | 30 | 50 |
| 49 | Advance Paychecks | $6.3M | 30 | 50 |
| 50 | Ultratech Tool & Design - Stamping | $6.0M | 30 | 50 |
| 51 | Fond Du Lac Truck Sales | $5.9M | 30 | 50 |
| 52 | diamond auto, inc | $5.7M | 30 | 50 |
| 53 | van horn hyundai of fond du lac | $5.7M | 30 | 50 |
| 54 | iAutomation (formerly PanelTEK LLC) | $5.7M | 30 | 50 |
| 55 | EP-Direct | $5.5M | 30 | 50 |
| 56 | Fat Joes Burgers and Brew | $5.1M | 125 | 50 |
| 57 | crescent lodging management inc | $5.1M | 125 | 50 |
| 58 | hotel retlaw, trademark collection by wyndham | $5.1M | 125 | 50 |
| 59 | fond du lac surgery center, llc | $5.0M | 30 | 50 |
| 60 | johnson chiropractic health service | $5.0M | 30 | 50 |
| 61 | Retlaw Plaza Hotel | $4.9M | 30 | 50 |
| 62 | Rademann Stone & Landscape Co., Inc. | $4.5M | 30 | 50 |
| 63 | Smith Builders Inc | $4.5M | 30 | 50 |
| 64 | jp tank, inc. | $4.5M | 30 | 50 |
| 65 | Guenther Supply Inc | $4.5M | 30 | 50 |
| 66 | burg homes & design, inc | $4.5M | 30 | 50 |
| 67 | Badger Metals Inc | $4.5M | 30 | 50 |
| 68 | binner pools, spas & fireplaces | $4.5M | 30 | 50 |
| 69 | LightPolesPlus.com | $4.5M | 30 | 50 |
| 70 | badgerland connections | $4.5M | 30 | 50 |
| 71 | Spies Painting Inc | $4.5M | 30 | 50 |
| 72 | fond du lac cyclery & fitness | $4.1M | 30 | 50 |
| 73 | dutch's outdoors llc | $4.1M | 30 | 50 |
| 74 | sparkops metalworks | $4.1M | 30 | 50 |
| 75 | MUTHIG INDUSTRIES, INC | $3.7M | 30 | 50 |
| 76 | Kelgor Inc | $3.5M | 30 | 50 |
| 77 | Kuddles College Llc | $3.4M | 30 | 50 |
| 78 | OTCO, Inc. | $3.1M | 30 | 50 |
| 79 | Crete-Heat, LLC. | $3.0M | 5 | 50 |
| 80 | journeyman machine & supply co inc | $2.9M | 30 | 50 |
| 81 | Cueburst | $2.8M | 30 | 50 |
| 82 | Amry Astro Group, Inc. | $2.8M | 30 | 50 |
| 83 | countryside auto & cycle | $2.8M | 30 | 50 |
| 84 | by grater whiter smiles | $2.8M | 30 | 50 |
| 85 | pro motor sports of fond du lac | $2.8M | 30 | 50 |
| 86 | matteson communications | $2.8M | 30 | 50 |
| 87 | rebath of central | $2.8M | 30 | 50 |
| 88 | sevice motor | $2.8M | 30 | 50 |
| 89 | Wrightway Inc | $2.6M | 30 | 50 |
| 90 | JMO Outdoors | $2.1M | 5 | 50 |
| 91 | hansen refrigeration service | $2.1M | 5 | 50 |
| 92 | Paramount Source | $2.0M | 5 | 50 |
| 93 | Dress for the Nine Maternity | $2.0M | 5 | 50 |
| 94 | Commah, LLC | $2.0M | 5 | 50 |
| 95 | Stitch Graphics | $2.0M | 5 | 50 |
| 96 | Evens Pest Control | $2.0M | 5 | 50 |
| 97 | Orth Enterprises Inc | $2.0M | 5 | 50 |
| 98 | snip shop | $2.0M | 5 | 50 |
| 99 | Best Engineering Company | $2.0M | 5 | 50 |
| 100 | Klapperich Real Estate Inc | $2.0M | 30 | 50 |