| 1 | BROWN & BROWN, INC. | $5.9B | -- | 85 |
| 2 | halifax hospital medical ctr | $3.3B | 20,000 | 80 |
| 3 | concrete legacy | $563.4M | 20,000 | 70 |
| 4 | DAYTONA BR-GD, INC. DBA DAYTONA TOYOTA | $142.8M | 350 | 70 |
| 5 | Brown & Brown Absence Services Group | $128.6M | 350 | 70 |
| 6 | Paxi Inc. | $96.0M | 350 | 60 |
| 7 | Suite Hospitality Staffing | $57.5M | 350 | 60 |
| 8 | GMC (General Mechanical Corporation) | $52.9M | 350 | 60 |
| 9 | US Patriot Logistics | $51.0M | 125 | 60 |
| 10 | Florida Department of Health in Volusia County | $35.2M | 350 | 60 |
| 11 | volusia county health dept | $35.2M | 350 | 60 |
| 12 | Atlas International Technology Services Inc | $33.1M | 350 | 60 |
| 13 | southeast automotive management, inc | $32.5M | 350 | 60 |
| 14 | Daytona Beverages, LLC | $30.8M | 125 | 60 |
| 15 | North American Lateral Flow ™ | $30.6M | 125 | 60 |
| 16 | DermaCare Research Lab, LLC | $27.4M | 125 | 60 |
| 17 | Thoroughbred Capital Partners | $26.3M | 125 | 60 |
| 18 | Floridian Bank | $26.3M | 125 | 60 |
| 19 | Robotics Association at Embry-Riddle | $25.0M | 125 | 60 |
| 20 | Daytona Dodge Chrysler Jeep & Ram | $24.8M | 125 | 60 |
| 21 | AutoSource Group | $24.8M | 125 | 60 |
| 22 | KART GROUP | $23.8M | 125 | 60 |
| 23 | Southeastern Lighting Solutions | $23.7M | 125 | 60 |
| 24 | Canepa Corporation | $23.0M | 30 | 60 |
| 25 | SCALA GLOBAL ACCELERATOR | $23.0M | 30 | 60 |
| 26 | Adams, Cameron & Co. Realtors | $22.9M | 350 | 60 |
| 27 | NEIGHBOR TO FAMILY, INC. | $21.3M | 125 | 60 |
| 28 | Hilton Daytona Beach Resort/Ocean Walk Village | $20.5M | 125 | 60 |
| 29 | coast insulation contractors inc | $18.9M | 125 | 60 |
| 30 | surface crafters, inc. | $18.9M | 125 | 60 |
| 31 | CARTER ELECTRIC CO., INC. | $18.9M | 125 | 60 |
| 32 | Vision General Contractors | $18.9M | 125 | 60 |
| 33 | Crane Cams | $15.3M | 125 | 60 |
| 34 | CMG Pro Security, Inc. | $14.4M | 125 | 60 |
| 35 | cook out east, inc | $14.3M | 350 | 60 |
| 36 | ERAU SHPE | $14.3M | 125 | 60 |
| 37 | O'Maley College of Business, Embry-Riddle Aeronautical University | $14.3M | 125 | 60 |
| 38 | embry-riddle/bethune-cookman ecocar team | $14.3M | 125 | 60 |
| 39 | Embry-Riddle Aeronautical University, Humanities and Communication Department | $14.3M | 125 | 60 |
| 40 | Americrown Services, LLC | $13.7M | 125 | 60 |
| 41 | Race Track Rv | $12.2M | 30 | 60 |
| 42 | Jessups Of Daytona Inc | $12.2M | 30 | 60 |
| 43 | Electronic Awards | $12.2M | 30 | 60 |
| 44 | Burns Supply Co | $12.2M | 30 | 60 |
| 45 | storms assorted nuts | $12.2M | 30 | 60 |
| 46 | Keyideas Infotech Private Limited | $11.1M | 125 | 60 |
| 47 | Silversphere / Sentrics Ensure360 | $11.1M | 125 | 60 |
| 48 | Employer Benefit Underwriters, Inc. | $11.0M | 30 | 60 |
| 49 | Commercial Coverages | $11.0M | 30 | 60 |
| 50 | ANJ STAFFING | $10.7M | 125 | 60 |
| 51 | vt designz | $9.9M | 350 | 50 |
| 52 | Atlantic Key Energy | $9.3M | 125 | 50 |
| 53 | Adsil | $8.7M | 30 | 50 |
| 54 | Trucklube 1 | $8.2M | 30 | 50 |
| 55 | Ctlc Inc | $8.2M | 125 | 50 |
| 56 | We Buy Houses Jacksonville FL | $8.2M | 125 | 50 |
| 57 | Continental Property Services, Inc. | $8.2M | 125 | 50 |
| 58 | RE/MAX Signature Daytona Beach Shores, Florida | $8.2M | 125 | 50 |
| 59 | DAYTONA BEACH (INC), CITY OF | $8.0M | 350 | 50 |
| 60 | all pro door & trim, inc. | $7.8M | 30 | 50 |
| 61 | all-florida plumbing & electrical supply co | $7.8M | 30 | 50 |
| 62 | Sly Fox Inn | $7.4M | 30 | 50 |
| 63 | Inlet Harbor Realty | $7.4M | 30 | 50 |
| 64 | TECHFIT Digital Surgery | Making digital surgery simple and seamless. | $7.3M | 30 | 50 |
| 65 | embry-riddle veteran student services, daytona beach campus | $7.0M | 750 | 50 |
| 66 | IEM Plastics | $6.9M | 30 | 50 |
| 67 | Scout Incentives | $6.8M | 30 | 50 |
| 68 | Indigo Manor Nursing & Rehabilitation | $6.7M | 125 | 50 |
| 69 | Forest Lake Manor | $6.7M | 125 | 50 |
| 70 | Indigo Manor | $6.7M | 125 | 50 |
| 71 | decorative electro coatings, inc. | $6.6M | 30 | 50 |
| 72 | braddock metallurgical aerospace services, inc. | $6.6M | 30 | 50 |
| 73 | JET GAMES CORPORATION | $6.6M | 30 | 50 |
| 74 | rama media | $6.5M | 350 | 50 |
| 75 | VOLUSIA/FLAGLER SHRM | $6.3M | 125 | 50 |
| 76 | Volusia County Bar Association Young Lawyers Division | $6.3M | 125 | 50 |
| 77 | AMBER GLOBE, INC. | $6.3M | 30 | 50 |
| 78 | FirsTrust, LLC | $6.3M | 30 | 50 |
| 79 | Evolution Financial Professionals | $6.3M | 30 | 50 |
| 80 | access to organics | $6.0M | 30 | 50 |
| 81 | Capannina Inc | $6.0M | 30 | 50 |
| 82 | angell and phelps | $6.0M | 30 | 50 |
| 83 | icy paradise | $6.0M | 30 | 50 |
| 84 | ERAU Women's Baja SAE Team | $5.9M | 30 | 50 |
| 85 | Hixon Motor Sports | $5.9M | 30 | 50 |
| 86 | Spanos Imports | $5.9M | 30 | 50 |
| 87 | Chuckran Auto Parts Inc | $5.7M | 30 | 50 |
| 88 | central florida auction block | $5.7M | 30 | 50 |
| 89 | Southern Power and Controls | $5.7M | 30 | 50 |
| 90 | Daytona Playhouse Live Theatre | $5.6M | 30 | 50 |
| 91 | Peninsular Printing | $5.5M | 30 | 50 |
| 92 | Checker Cabs | $5.4M | 30 | 50 |
| 93 | Container Systems & Equipment | $5.4M | 30 | 50 |
| 94 | GEEK PATCH | $5.1M | 30 | 50 |
| 95 | Colleen AI | $5.1M | 30 | 50 |
| 96 | Dales Shoes and Pedorthics | $5.1M | 30 | 50 |
| 97 | the arc volusia | $5.1M | 125 | 50 |
| 98 | streamline hotel - daytona beach | $5.1M | 125 | 50 |
| 99 | silver beach club ltd., inc. | $5.1M | 125 | 50 |
| 100 | tomoka periodontics and implant dentistry, p.a | $5.0M | 30 | 50 |