| 1 | youngglobaltech | $161.0M | 20,000 | 70 |
| 2 | WYATT JOHNSON BUICK, PONTIAC, GMC TRUCK, INC | $142.8M | 350 | 70 |
| 3 | GOODWILL OF SOUTHERN INDIANA INC | $142.8M | 350 | 70 |
| 4 | CDE Lightband | $96.0M | 350 | 60 |
| 5 | apsu international | $85.8M | 750 | 60 |
| 6 | dbs & associates engineering, inc. | $69.3M | 350 | 60 |
| 7 | BRIDGESTONE METALPHA USA, INC | $66.5M | 350 | 60 |
| 8 | Coyle Chevrolet Buick GMC | $51.0M | 125 | 60 |
| 9 | Mumbai Boutique LLC, Clarksville, MD, USA | $51.0M | 125 | 60 |
| 10 | Wireless Direct | $51.0M | 125 | 60 |
| 11 | Cumberland Electric Membership Corporation, Inc. | $44.9M | 350 | 60 |
| 12 | Austin Peay State University College of STEM | $40.1M | 350 | 60 |
| 13 | D2 ENERGY LLC | $38.1M | 30 | 60 |
| 14 | CLARKSVILLE TRANSIT SYSTEM | $34.3M | 125 | 60 |
| 15 | MPG Companies, Inc. | $34.3M | 125 | 60 |
| 16 | Clean Eatz Clarksville TN | $30.8M | 125 | 60 |
| 17 | AJAX DISTRIBUTING COMPANY by zaher nourredine | $30.8M | 125 | 60 |
| 18 | First Savings Bank, Indiana | $26.3M | 125 | 60 |
| 19 | Diatex USA | $25.0M | 125 | 60 |
| 20 | SMIC,INC | $25.0M | 125 | 60 |
| 21 | Golly G's: Coffee, Ice Cream, & Sweets | $24.9M | 125 | 60 |
| 22 | Aguire International, Inc. | $24.8M | 125 | 60 |
| 23 | JENKINS & WYNNE, INC. | $23.8M | 125 | 60 |
| 24 | Facility Services Inc. | $21.8M | 350 | 60 |
| 25 | Facility Services Managemenmt Inc (FSI) | $21.8M | 350 | 60 |
| 26 | Brightwell Behavioral Health | $20.9M | 125 | 60 |
| 27 | Dan Cristiani Excavating Inc | $18.9M | 125 | 60 |
| 28 | Classic Truss and Wood Components, Inc | $18.9M | 125 | 60 |
| 29 | jcm management, inc | $18.9M | 125 | 60 |
| 30 | Amec Civil LLC | $18.9M | 125 | 60 |
| 31 | SR Wood, Inc. | $17.8M | 30 | 60 |
| 32 | mw/mb, llc | $17.0M | 125 | 60 |
| 33 | ECHO Power Engineering | $16.0M | 125 | 60 |
| 34 | sawhney industries | $15.3M | 125 | 60 |
| 35 | Freestate Wellness, LLC | $15.3M | 30 | 60 |
| 36 | VVS Partners LLC | $14.6M | 125 | 60 |
| 37 | Vonalas, Inc | $14.6M | 125 | 60 |
| 38 | International Students Organization Austin Peay State University | $14.3M | 125 | 60 |
| 39 | United States Postal Services | $12.6M | 125 | 60 |
| 40 | Probation Parole Svc | $12.6M | 125 | 60 |
| 41 | Choice Concrete Construction | $12.5M | 30 | 60 |
| 42 | Farm Service Store Family Collective | $12.4M | 30 | 60 |
| 43 | Tom Stinnett Holiday RV Center Inc. | $12.2M | 30 | 60 |
| 44 | Wyatt Johnson Buick GMC | $12.2M | 30 | 60 |
| 45 | Jaguar Land Rover of West Columbia | $12.2M | 30 | 60 |
| 46 | DIRECT AUTO OF TENNESSEE, LLC | $12.2M | 30 | 60 |
| 47 | Patriotic Fireworks Inc | $12.2M | 30 | 60 |
| 48 | Kidz Concepts | $12.2M | 30 | 60 |
| 49 | World Outdoor Resources, LLC | $12.2M | 30 | 60 |
| 50 | MOORE'S CHEVROLET INC. | $12.2M | 30 | 60 |
| 51 | Dollar Tree Stores Inc 2336 | $12.2M | 30 | 60 |
| 52 | Wyatt Johnson Mazda | $12.2M | 30 | 60 |
| 53 | Dodges Stores | $12.2M | 30 | 60 |
| 54 | BRIDGEPOINTE GOODWILL | $12.2M | 30 | 60 |
| 55 | Clarksville Floor Covering Co | $12.2M | 30 | 60 |
| 56 | Mildred & Mable's, Inc. | $12.2M | 30 | 60 |
| 57 | FBC Logistic | $12.2M | 30 | 60 |
| 58 | gallardo’s cakes and bakery | $12.2M | 30 | 60 |
| 59 | nerdy birdy cookie company | $12.2M | 30 | 60 |
| 60 | mpg transport, inc. | $12.0M | 125 | 60 |
| 61 | Bell Electrical Service | $11.8M | 30 | 60 |
| 62 | ACTIVE ENERGY SERVICES, LLC | $11.8M | 30 | 60 |
| 63 | Insurance Company of America | $11.0M | 30 | 60 |
| 64 | Tennessee Farmers Mutual | $11.0M | 30 | 60 |
| 65 | Clarksville Oil & Gas Company | $9.4M | 30 | 50 |
| 66 | looks matter landscaping | $9.3M | 125 | 50 |
| 67 | ramnova tech | $8.8M | 125 | 50 |
| 68 | Sodrel Transportation Group | $8.2M | 30 | 50 |
| 69 | Coldwell Banker Conroy Marable & Holleman | $8.2M | 125 | 50 |
| 70 | the creig northrop team of long & foster real estate, inc | $8.2M | 125 | 50 |
| 71 | Amylex📍Maryland Realtor | Certified Negotiations Expert👩🏽💼👑 | $8.2M | 125 | 50 |
| 72 | Red Ball Recycling, LLC | $8.0M | 30 | 50 |
| 73 | H&H Truss and supply | $7.8M | 30 | 50 |
| 74 | Lakeshore Beverage Co | $7.4M | 30 | 50 |
| 75 | Scoop and Paddle Ice Cream Company | $7.4M | 30 | 50 |
| 76 | Oncologix Corporation | $7.3M | 30 | 50 |
| 77 | Insightra Medical Inc | $7.3M | 30 | 50 |
| 78 | GHK Truss LLC | $7.1M | 30 | 50 |
| 79 | CLARKSVILLE FOUNDRY, INC. | $7.1M | 30 | 50 |
| 80 | CLARKSVILLE COMM SCHOOL DISTRICT | $6.9M | 125 | 50 |
| 81 | red river machine llc | $6.8M | 30 | 50 |
| 82 | GeeseMed | $6.7M | 125 | 50 |
| 83 | PAM Health Rehabilitation Hospital of Greater Indiana | $6.7M | 125 | 50 |
| 84 | Triumph Nursing Staff | $6.7M | 125 | 50 |
| 85 | Arcadia Senior Living Clarksville | $6.7M | 125 | 50 |
| 86 | Magnolia Brush Mfrs Inc | $6.6M | 30 | 50 |
| 87 | ARMS ROOM OUTFITTERS LLC | $6.6M | 30 | 50 |
| 88 | facility services managemenmt inc (fsi) | $6.4M | 350 | 50 |
| 89 | Jack B. Turner and Associates | $6.3M | 30 | 50 |
| 90 | Stone Rudolph & Henry, PLC | $6.3M | 30 | 50 |
| 91 | Iowa State Bank (Clarksville, IA) | $6.3M | 30 | 50 |
| 92 | Aaa Industries | $6.0M | 30 | 50 |
| 93 | B O C Gasses | $6.0M | 30 | 50 |
| 94 | nora’s filipino market | $6.0M | 30 | 50 |
| 95 | the star spangled brewing company | $6.0M | 30 | 50 |
| 96 | Ourisman Chrysler, Dodge, Jeep, Ram | $5.9M | 30 | 50 |
| 97 | Coyle Dodge Inc | $5.9M | 30 | 50 |
| 98 | Antwerpen Nissan | $5.9M | 30 | 50 |
| 99 | Jim Butner Auto Inc | $5.7M | 30 | 50 |
| 100 | Mid South Motoplex | $5.7M | 30 | 50 |