| 1 | collegiate sales excellence | $2.3B | 20,000 | 80 |
| 2 | ashland university graduate, international, and online admissions | $343.3M | 3,000 | 70 |
| 3 | GREEN GROUP TRUCKING | $96.0M | 350 | 60 |
| 4 | Chick-fil-A Center of the Universe & Virginia Center Marketplace | $86.3M | 350 | 60 |
| 5 | ashland ind school district | $85.8M | 750 | 60 |
| 6 | Atlas Bolt & Screw Company | $74.0M | 125 | 60 |
| 7 | resource international, ltd. | $69.3M | 350 | 60 |
| 8 | ARM Resource | $69.3M | 350 | 60 |
| 9 | Anton Paar USA | $66.5M | 350 | 60 |
| 10 | Neuman Hotel Group | $57.5M | 350 | 60 |
| 11 | Pavement Partners Holding | $52.9M | 350 | 60 |
| 12 | TC CHEVROLET INC | $51.0M | 125 | 60 |
| 13 | Smarter Tools, Inc. | $51.0M | 125 | 60 |
| 14 | Green Top Sporting Goods Corp / Green Top Shooting Range | $51.0M | 125 | 60 |
| 15 | Orbit Healthcare | $51.0M | 125 | 60 |
| 16 | Orbit Same Day | $51.0M | 125 | 60 |
| 17 | Workers Comp Insurance | $45.9M | 125 | 60 |
| 18 | Flexicell, A Pearson Packaging Systems Company | $45.0M | 125 | 60 |
| 19 | El Mar Inc | $41.1M | 30 | 60 |
| 20 | Ashland University Center for Addictions | $40.1M | 350 | 60 |
| 21 | Eagle Community Partners | $40.1M | 350 | 60 |
| 22 | JRP (James River Petroleum) | $39.2M | 125 | 60 |
| 23 | Ashland Foundry & Machine Works LLC | $39.2M | 125 | 60 |
| 24 | Town & Country Co-op, Inc. | $39.2M | 125 | 60 |
| 25 | City of Ashland Kentucky | $35.2M | 350 | 60 |
| 26 | City of Ashland, OR | $35.2M | 350 | 60 |
| 27 | Utility Trailer Sales-Virginia | $34.3M | 125 | 60 |
| 28 | ADDCAR International ADDCAR America | $33.3M | 125 | 60 |
| 29 | Home Lumber and Supply Company | $32.4M | 125 | 60 |
| 30 | tru cabinetry | $32.4M | 125 | 60 |
| 31 | Cropper Medical Inc / Pro Bio Skin | $30.6M | 125 | 60 |
| 32 | BioSkin - Innovative Bracing Solutions | $30.6M | 125 | 60 |
| 33 | C G Bretting Manufacturing Co | $29.2M | 125 | 60 |
| 34 | Americarb Corporation | $29.2M | 125 | 60 |
| 35 | Hughes Tool | $28.2M | 125 | 60 |
| 36 | Chandler Systems Inc | $27.4M | 125 | 60 |
| 37 | Blue Collar Life Group | $26.3M | 125 | 60 |
| 38 | Members Choice Credit Union (Ashland, KY) | $26.3M | 125 | 60 |
| 39 | Northern State Bank (Ashland, WI) | $26.3M | 125 | 60 |
| 40 | Town Square Bank, Inc. | $26.3M | 125 | 60 |
| 41 | West Central Bank (Ashland, IL) | $26.3M | 125 | 60 |
| 42 | standing stone brewing co | $24.9M | 125 | 60 |
| 43 | s & j reed, inc | $24.9M | 125 | 60 |
| 44 | Butler Automotive Group (Acura, Ford, Hyundai, Kia) | $24.8M | 125 | 60 |
| 45 | Donley Auto Group | $24.8M | 125 | 60 |
| 46 | Car Wash Superstore | $24.8M | 125 | 60 |
| 47 | don hall gm supercenter | $23.8M | 125 | 60 |
| 48 | A Better VA | $22.9M | 350 | 60 |
| 49 | betterpsych inc. | $20.9M | 125 | 60 |
| 50 | St. Labre Indian School | $19.2M | 350 | 60 |
| 51 | stanley construction company., inc. | $18.9M | 125 | 60 |
| 52 | F. Richard Wilton | $18.9M | 125 | 60 |
| 53 | Southwood Building Systems, Inc. | $18.9M | 125 | 60 |
| 54 | Simonson Construction Services, Inc. | $18.9M | 125 | 60 |
| 55 | New Field Inc. | $18.9M | 125 | 60 |
| 56 | Green Air, Inc | $18.9M | 125 | 60 |
| 57 | twc phoenix | $18.9M | 125 | 60 |
| 58 | Architectural Products of Virginia (APV) | $18.9M | 125 | 60 |
| 59 | Ashland Lumber Company Inc. | $17.8M | 30 | 60 |
| 60 | Kennedy Concrete, Inc | $17.8M | 30 | 60 |
| 61 | Carlson Building Supplies, Inc. | $17.8M | 30 | 60 |
| 62 | ironcrafters security products, inc. | $17.0M | 125 | 60 |
| 63 | greenflame | $17.0M | 125 | 60 |
| 64 | t.j. wolf, inc. | $14.3M | 350 | 60 |
| 65 | a-c central cusd 262 school district | $14.3M | 125 | 60 |
| 66 | purchasepros | $14.3M | 125 | 60 |
| 67 | Ashland University Correctional Education | $14.3M | 125 | 60 |
| 68 | Vitl Life Science Solutions | $13.0M | 125 | 60 |
| 69 | Town of Ashland, Virginia | $12.6M | 125 | 60 |
| 70 | Lute Plumbing Supply | $12.4M | 30 | 60 |
| 71 | Ashland Pump | $12.4M | 30 | 60 |
| 72 | general sales company of ashland, incorporated | $12.4M | 30 | 60 |
| 73 | All’s Well Herb & Vitamin Shop | $12.2M | 30 | 60 |
| 74 | Ashland Feed Store, LLC | $12.2M | 30 | 60 |
| 75 | Paddington Family of Stores | $12.2M | 30 | 60 |
| 76 | Personalization Inc. | $12.2M | 30 | 60 |
| 77 | Progress Wire Products Inc | $12.2M | 30 | 60 |
| 78 | Aladdin’s Art Gallery Inc | $12.2M | 30 | 60 |
| 79 | Summit + Fields | $12.2M | 30 | 60 |
| 80 | ROCHESTER SHOE TREE COMPANY | $12.2M | 30 | 60 |
| 81 | East Coast Wine Fulfillment | $12.2M | 30 | 60 |
| 82 | sunstone artisan bakery | $12.2M | 30 | 60 |
| 83 | gerwig’s white barn market | $12.2M | 30 | 60 |
| 84 | belle fiore | $12.2M | 30 | 60 |
| 85 | sweet desire vegan bakery & confectionary | $12.2M | 30 | 60 |
| 86 | RVA CONSTRUCTION, INC. | $11.8M | 30 | 60 |
| 87 | Herring’s Roofing and Painting | $11.8M | 30 | 60 |
| 88 | donley ford lincoln of ashland | $11.6M | 125 | 60 |
| 89 | mcgeorge camping center inc | $11.6M | 125 | 60 |
| 90 | donley ford-lincoln, inc | $11.6M | 125 | 60 |
| 91 | NPA Return-to-Work Services | $11.0M | 30 | 60 |
| 92 | Sturtevant Associates | $11.0M | 30 | 60 |
| 93 | L B S Insurance | $11.0M | 30 | 60 |
| 94 | Magnum Metals - Industrial Automation | $10.8M | 30 | 60 |
| 95 | Automatic Controls of Virginia, Inc. | $10.8M | 30 | 60 |
| 96 | sky research | $9.3M | 125 | 50 |
| 97 | BCLS Snow and Ice Management | $9.3M | 125 | 50 |
| 98 | Ashland Plastics Inc | $8.7M | 30 | 50 |
| 99 | Leaf Home Enhancements | $8.5M | 125 | 50 |
| 100 | Royster Enterprises | $8.2M | 30 | 50 |