| 1 | WORKIVA INC | €884.6M | -- | 80 | A |
| 2 | AMES NATIONAL CORPORATION | €87.1M | -- | 80 | A |
| 3 | WG Provisions Biomedical | €51.5M | 125 | 60 | C+ |
| 4 | Wheatsfield Co-op | €51.0M | 125 | 60 | C+ |
| 5 | COUNTRY LANDSCAPES, INC. | €49.0M | 125 | 60 | C+ |
| 6 | Department of Animal Science - Iowa State University | €40.1M | 350 | 60 | C+ |
| 7 | Iowa State University School of Education | €40.1M | 350 | 60 | C+ |
| 8 | Iowa State University - Academic Success Center | €40.1M | 350 | 60 | C+ |
| 9 | Iowa State University - College of Agriculture and Life Sciences | €40.1M | 350 | 60 | C+ |
| 10 | Iowa State University - Educational Technology Emphasis Program | €40.1M | 350 | 60 | C+ |
| 11 | Student Innovation Center - Iowa State University | €40.1M | 350 | 60 | C+ |
| 12 | Iowa State University College of Human Sciences | €40.1M | 350 | 60 | C+ |
| 13 | Hawkeye Energy Holdings, LLC | €39.2M | 125 | 60 | C+ |
| 14 | Center for Biorenewable Chemicals (CBiRC) | €36.2M | 125 | 60 | C+ |
| 15 | TRANSPORTATION EXPRESS INC | €34.3M | 125 | 60 | C+ |
| 16 | Beacon Microcenter Inc | €31.1M | 350 | 60 | C+ |
| 17 | Burgies Coffee & Tea Company | €30.8M | 125 | 60 | C+ |
| 18 | Cardinal Space Mining | €29.2M | 125 | 60 | C+ |
| 19 | go-ecpe at iowa state university | €28.0M | 3,000 | 60 | C+ |
| 20 | iowa state athletes | €28.0M | 3,000 | 60 | C+ |
| 21 | Ogden BancShares, Inc. | €26.3M | 125 | 60 | C+ |
| 22 | Iowa State University Investment Group | €26.3M | 125 | 60 | C+ |
| 23 | PrISUm Solar Car | €24.8M | 125 | 60 | C+ |
| 24 | Wilson Cadillac Toyota Scion | €24.8M | 125 | 60 | C+ |
| 25 | institute of transportation engineers, isu chapter (tsa) | €24.8M | 125 | 60 | C+ |
| 26 | formula sae at iowa state | €23.8M | 125 | 60 | C+ |
| 27 | Iowa State Daily Media Group | €23.1M | 350 | 60 | C+ |
| 28 | iowa state men's hockey club | €20.3M | 125 | 60 | C+ |
| 29 | lakeside contractors | €18.9M | 125 | 60 | C+ |
| 30 | ACI MECHANICAL INC. | €18.9M | 125 | 60 | C+ |
| 31 | Nelson Electric Company, LC | €18.9M | 125 | 60 | C+ |
| 32 | Munn Lumber | €17.8M | 30 | 60 | C+ |
| 33 | City of Ames Electric Services | €16.0M | 125 | 60 | C+ |
| 34 | Women Who Design @Iowa State | €15.7M | 125 | 60 | C+ |
| 35 | Iowa State University Department of Aerospace Engineering | €14.3M | 125 | 60 | C+ |
| 36 | Iowa State University - Ivy College of Business | €14.3M | 125 | 60 | C+ |
| 37 | Department of Psychology - Iowa State | €14.3M | 125 | 60 | C+ |
| 38 | Iowa State University Department of Chemical and Biological Engineering | €14.3M | 125 | 60 | C+ |
| 39 | Iowa-Beta Sigma Phi Epsilon | €14.3M | 125 | 60 | C+ |
| 40 | ISU Department of Statistics | €14.3M | 125 | 60 | C+ |
| 41 | ISU Department of Civil, Construction and Environmental Engineering | €14.3M | 125 | 60 | C+ |
| 42 | TrAC - Translational AI Center | €14.3M | 125 | 60 | C+ |
| 43 | Game2Work | €14.3M | 125 | 60 | C+ |
| 44 | Iowa State Chapter of FarmHouse Fraternity | €14.3M | 125 | 60 | C+ |
| 45 | Kappa Sigma Fraternity at Iowa State University | €14.3M | 125 | 60 | C+ |
| 46 | National Student Exchange | €14.3M | 125 | 60 | C+ |
| 47 | ISU Department of Computer Science | €14.3M | 125 | 60 | C+ |
| 48 | Iowa State University Department of Agricultural and Biosystems Engineering | €14.3M | 125 | 60 | C+ |
| 49 | Iowa State University Food Science & Human Nutrition Department | €14.3M | 125 | 60 | C+ |
| 50 | Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology at Iowa State University | €14.3M | 125 | 60 | C+ |
| 51 | Iowa State University Department of Electrical and Computer Engineering | €13.0M | 125 | 60 | C+ |
| 52 | Center for Nondestructive Evaluation | €13.0M | 125 | 60 | C+ |
| 53 | The Fashion Show | €12.4M | 125 | 60 | C+ |
| 54 | A&R Enterprises L.L.C. | €12.2M | 30 | 60 | C+ |
| 55 | Cyclone Liquors | €12.2M | 30 | 60 | C+ |
| 56 | Ames Racquet & Fitness | €12.2M | 30 | 60 | C+ |
| 57 | caremoli usa inc. | €12.2M | 30 | 60 | C+ |
| 58 | ali cakes, llc | €12.2M | 30 | 60 | C+ |
| 59 | greenika skin care | €11.6M | 125 | 60 | C+ |
| 60 | BEST WESTERN UNIVERSITY PARK INN AND SUITES | €11.0M | 30 | 60 | C+ |
| 61 | American Measurement Svc | €9.4M | 30 | 50 | C |
| 62 | C and K Inc. | €8.5M | 125 | 50 | C |
| 63 | phClean ~ Maid Service | €8.5M | 125 | 50 | C |
| 64 | Md Transport | €8.2M | 30 | 50 | C |
| 65 | Conley's Trucking, Inc | €8.2M | 30 | 50 | C |
| 66 | Iowa State University Athletics Department | €8.0M | 350 | 50 | C |
| 67 | Sargent Metal Fabricating | €8.0M | 30 | 50 | C |
| 68 | keith cooper & sons inc | €7.8M | 30 | 50 | C |
| 69 | iowa state university, college of engineering-human resources | €7.0M | 750 | 50 | C |
| 70 | Green Hills Community | €6.7M | 125 | 50 | C |
| 71 | CFG Promos | €6.6M | 30 | 50 | C |
| 72 | Partners for Production Agriculture | €6.3M | 30 | 50 | C |
| 73 | Iowa State University Wall Street Club | €6.3M | 30 | 50 | C |
| 74 | PeachPay HQ | €6.3M | 30 | 50 | C |
| 75 | Country Plastics | €6.0M | 30 | 50 | C |
| 76 | Iowa State Daily | €6.0M | 51 | 50 | C |
| 77 | olde main brewing co. | €6.0M | 30 | 50 | C |
| 78 | The Joan Bice Underwood Tearoom at Iowa State University | €6.0M | 30 | 50 | C |
| 79 | Original Appearance Manufacturing | €5.9M | 30 | 50 | C |
| 80 | Monarch Defender | €5.9M | 30 | 50 | C |
| 81 | Sigma Gamma Tau- Iowa State University | €5.2M | 30 | 50 | C |
| 82 | ASPI Solutions Inc | €5.1M | 30 | 50 | C |
| 83 | EasyView USA | €5.1M | 30 | 50 | C |
| 84 | Jet Print | €5.1M | 30 | 50 | C |
| 85 | northcrest health care center | €5.0M | 30 | 50 | C |
| 86 | jorgensen chiropractic pc | €5.0M | 30 | 50 | C |
| 87 | mary greeley homeward hospice & israel | €5.0M | 30 | 50 | C |
| 88 | Ames & West Des Moines Therapy & Consulting | €5.0M | 30 | 50 | C |
| 89 | readhead paul dds | €5.0M | 30 | 50 | C |
| 90 | five oaks chiropractic and acupuncture | €5.0M | 30 | 50 | C |
| 91 | accelerated freefall iowa, inc. | €5.0M | 30 | 50 | C |
| 92 | mcfarland clinic pc - medical arts | €5.0M | 30 | 50 | C |
| 93 | Jaspering Electric | €4.5M | 30 | 50 | C |
| 94 | Mechanical Comfort | €4.5M | 30 | 50 | C |
| 95 | brandon training | €4.5M | 30 | 50 | C |
| 96 | hcs builders inc | €4.5M | 30 | 50 | C |
| 97 | ServiceMaster of Story & Boone County | €4.5M | 30 | 50 | C |
| 98 | Harold Pike Construction Co | €4.5M | 30 | 50 | C |
| 99 | hutson-thompson plumbing inc. | €4.5M | 30 | 50 | C |
| 100 | ames trenching & excavating inc | €4.5M | 30 | 50 | C |