| 1 | Carloft | €24.8M | 125 | 60 | C+ |
| 2 | Kingston Market Square Hotel | €20.5M | 125 | 60 | C+ |
| 3 | Queen's BioMechatronics Team | €15.3M | 125 | 60 | C+ |
| 4 | Queen's University - School of Policy Studies | €14.3M | 125 | 60 | C+ |
| 5 | ECEi at Queen's University | €14.3M | 125 | 60 | C+ |
| 6 | Queen's Ophthalmology | €14.3M | 125 | 60 | C+ |
| 7 | Queen's Hyperloop Design Team | €13.0M | 125 | 60 | C+ |
| 8 | PREVNet - Promoting Relationships and Eliminating Violence Network | €13.0M | 125 | 60 | C+ |
| 9 | nidus3d | €11.8M | 30 | 60 | C+ |
| 10 | Queen's Actuarial Club (QAC) | €11.0M | 30 | 60 | C+ |
| 11 | guardsman insurance services inc. | €11.0M | 30 | 60 | C+ |
| 12 | Kingston Toyota | €9.2M | 30 | 50 | C |
| 13 | queen's supermileage | €9.2M | 30 | 50 | C |
| 14 | amaryllis technology group | €8.8M | 125 | 50 | C |
| 15 | renewable energy of plum hollow inc. | €8.5M | 30 | 50 | C |
| 16 | Keilty Realty Corporation | €8.2M | 125 | 50 | C |
| 17 | DeCoste Manufacturing Ltd. | €7.2M | 30 | 50 | C |
| 18 | Muse Research Inc. | €6.8M | 5 | 50 | C |
| 19 | mine design technologies | €6.6M | 30 | 50 | C |
| 20 | Next Horizon Program | €6.3M | 125 | 50 | C |
| 21 | Secura Wish | €6.3M | 30 | 50 | C |
| 22 | analyst insights | €6.3M | 30 | 50 | C |
| 23 | javarite | €6.0M | 30 | 50 | C |
| 24 | Queen's Global Innovation Conference | €5.9M | 30 | 50 | C |
| 25 | Colliding Scopes Theatre Company | €5.6M | 30 | 50 | C |
| 26 | Theia Markerless | €5.1M | 30 | 50 | C |
| 27 | Queen's Technology & Media Association | €5.1M | 30 | 50 | C |
| 28 | Garrison Golf & Curling Club | €4.9M | 30 | 50 | C |
| 29 | distributivelabs | €3.9M | 30 | 50 | C |
| 30 | server cloud canada | €3.9M | 30 | 50 | C |
| 31 | GISC Global Innovations | €3.8M | 5 | 50 | C |
| 32 | Agro Maritime Commodities | €3.8M | 5 | 50 | C |
| 33 | Yupi.ca | €3.8M | 30 | 50 | C |
| 34 | queen’s university irc | €3.4M | 30 | 50 | C |
| 35 | queen's university optica student chapter | €3.4M | 30 | 50 | C |
| 36 | Centre for Social Impact | Smith School of Business | €3.4M | 30 | 50 | C |
| 37 | inter-collegiate business competition (i.c.b.c.) | €3.4M | 30 | 50 | C |
| 38 | rokeby educational trust limited | €3.4M | 30 | 50 | C |
| 39 | Queen's Women in Leadership – QWIL | €3.4M | 30 | 50 | C |
| 40 | smith business international students association | €3.4M | 30 | 50 | C |
| 41 | Conference on Industry and Resources: Queen's University Engineering | €3.4M | 30 | 50 | C |
| 42 | MBA Legacy Fund at Smith School of Business | €3.4M | 30 | 50 | C |
| 43 | exchange and transfer committee (etc) | €3.3M | 30 | 50 | C |
| 44 | QMIND | €3.1M | 350 | 50 | C |
| 45 | Millenium Biologix Corp | €3.1M | 30 | 50 | C |
| 46 | Queen's Themed Entertainment Development Team | €3.1M | 30 | 50 | C |
| 47 | queen's universities allied for essential medicines | €3.0M | 30 | 50 | C |
| 48 | hardstaff traffic barrier services limited | €2.9M | 30 | 50 | C |
| 49 | Mesh AI (collaborative scheduling to end clinician burnout) | €2.8M | 30 | 50 | C |
| 50 | allCare IT | €2.7M | 30 | 50 | C |
| 51 | Queen's Human Capital | €2.6M | 30 | 50 | C |
| 52 | ozira foods | €2.3M | 5 | 50 | C |
| 53 | Queen's Carbon Capture and Conversion Team (QCCC) | €2.2M | 30 | 50 | C |
| 54 | bagot leather goods, luggage plus | €2.0M | 5 | 50 | C |
| 55 | Qtradition Inc. | €2.0M | 5 | 50 | C |
| 56 | B&H WHOLESALERS/CLOTHING & PROMOTIONAL | €2.0M | 5 | 50 | C |
| 57 | Bread By Élise | €2.0M | 5 | 50 | C |
| 58 | kelly's gourmet mushrooms | €2.0M | 5 | 50 | C |
| 59 | Alwington Communities | €2.0M | 30 | 50 | C |
| 60 | Commercial Source | €2.0M | 30 | 50 | C |
| 61 | Queen's Real Estate Investment Association | €2.0M | 30 | 50 | C |
| 62 | Drake's Landscaping | €1.9M | 30 | 50 | C |
| 63 | the mortgage professionals (verico) | €1.8M | 30 | 50 | C |
| 64 | queen's university investment counsel | €1.7M | 30 | 50 | C |
| 65 | Girls SySTEM Mentorship Program | €1.6M | 30 | 50 | C |
| 66 | msann | €1.6M | 5 | 50 | C |
| 67 | FALC0N-X | €1.6M | 5 | 50 | C |
| 68 | TotalHIRE | €1.5M | 30 | 50 | C |
| 69 | Aitken Robertson Criminal & DUI Defence | €1.5M | 30 | 50 | C |
| 70 | aitken robertson criminal lawyers | €1.5M | 30 | 50 | C |
| 71 | viner, kennedy, frederick, allan & tobias llp | €1.5M | 30 | 50 | C |
| 72 | CO2L Technologies Inc. | €1.4M | 5 | 50 | C |
| 73 | Blue Streak Express | €1.4M | 5 | 50 | C |
| 74 | Focus Graphite | €1.3M | 5 | 50 | C |
| 75 | APMT | €1.3M | 5 | 50 | C |
| 76 | Yieldpoint Inc. | €1.3M | 5 | 50 | C |
| 77 | Hydroclave North America | €1.2M | 5 | 50 | C |
| 78 | BGC South East | €1.2M | 125 | 50 | C |
| 79 | Kribr Sustainable Innovations Inc | €1.1M | 5 | 50 | C |
| 80 | Trellis HIV & Community Care (formerly HARS) | €1.1M | 125 | 50 | C |
| 81 | GORWAY / YESCO Ontario East | €1.1M | 5 | 50 | C |
| 82 | Awake Scrubs | €1.1M | 5 | 50 | C |
| 83 | path2zero inc. | €1.1M | 5 | 50 | C |
| 84 | Strategic Benefits & Insurance Services Ltd. | €1.1M | 5 | 50 | C |
| 85 | Advocis Kingston | €1.1M | 5 | 50 | C |
| 86 | Laura Southall CFP®, CLU® | €1.1M | 5 | 50 | C |
| 87 | Agro Merchant Bank | €1.1M | 5 | 50 | C |
| 88 | Alpha Accelerator Investment Management (AAIM) | €1.1M | 5 | 50 | C |
| 89 | Hartzman & Associates Inc | €1.1M | 5 | 50 | C |
| 90 | kingston anchors marine products inc. | €987K | 5 | 40 | D |
| 91 | 5th Company Lane | €929K | 5 | 40 | D |
| 92 | delivering customers | €923K | 5 | 40 | D |
| 93 | ablaze solutions | €923K | 5 | 40 | D |
| 94 | KKP Kingston - Design, Print & Business Solutions | €917K | 5 | 40 | D |
| 95 | Glover & Boos | €851K | 5 | 40 | D |
| 96 | SWT Analytics | €851K | 5 | 40 | D |
| 97 | Tripz Systems Inc. | €851K | 5 | 40 | D |
| 98 | inField | €851K | 5 | 40 | D |
| 99 | Queen's Data Science Club | €851K | 5 | 40 | D |
| 100 | Wavve Boating Inc. | €851K | 5 | 40 | D |