| 1 | NS Groep N.V. | €3.5B | 21,369 | 70 |
| 2 | bam wegen | €594.1M | 3,000 | 70 |
| 3 | idh, the sustainable trade initiative | €268.2M | 350 | 70 |
| 4 | FAAC Automatische Deuren | €207.2M | 350 | 70 |
| 5 | equensworldline | €179.7M | 3,000 | 70 |
| 6 | Zorgapotheek Nederland B.V. | €101.2M | 350 | 70 |
| 7 | Sociëteit "De Vereeniging" Utrecht | €99.4M | 350 | 60 |
| 8 | wind & water works | €95.8M | 125 | 60 |
| 9 | Dominican & European Chamber of Commerce | €95.8M | 125 | 60 |
| 10 | ulex llp | €71.8M | 750 | 60 |
| 11 | yoghurt barn | €69.6M | 350 | 60 |
| 12 | direct dial | €64.6M | 350 | 60 |
| 13 | VersTrade | €63.5M | 125 | 60 |
| 14 | Van der Wal B.V. | €62.9M | 350 | 60 |
| 15 | chateau viande utrecht | €57.7M | 125 | 60 |
| 16 | VSKbeurs | €52.9M | 350 | 60 |
| 17 | Strukton Bouw en Vastgoed | €52.9M | 350 | 60 |
| 18 | Dura Vermeer Divisie Techniek | €52.9M | 350 | 60 |
| 19 | Eurail.com | €51.0M | 125 | 60 |
| 20 | Pharma Chemicals NL | €50.4M | 350 | 60 |
| 21 | axyware (a lumera company) | €45.1M | 350 | 60 |
| 22 | Becoming Alliance | €44.9M | 350 | 60 |
| 23 | Customer Care the Voice | €40.9M | 350 | 60 |
| 24 | Tankstation Vakbeurs 2023 | €39.2M | 125 | 60 |
| 25 | Vakbeurs Elektrotechniek | €38.4M | 350 | 60 |
| 26 | Lustrum Odysseus | €38.4M | 350 | 60 |
| 27 | g. van doorn papier recycling utrecht b.v. | €38.1M | 30 | 60 |
| 28 | BPUG | €36.4M | 350 | 60 |
| 29 | Fashion4 | €34.6M | 350 | 60 |
| 30 | Rail_KIVI | €34.3M | 125 | 60 |
| 31 | 2Getthere B.V. | €34.3M | 125 | 60 |
| 32 | willemsen infra bv | €34.3M | 125 | 60 |
| 33 | Previder | Corporaties | €33.1M | 350 | 60 |
| 34 | Telindus Nederland | €33.1M | 350 | 60 |
| 35 | Axxes IT Consultancy Nederland | €31.1M | 350 | 60 |
| 36 | Abran Oils | €30.8M | 125 | 60 |
| 37 | Azbil Telstar Benelux B.V. | €29.2M | 125 | 60 |
| 38 | Wattkraft Solar Benelux | €28.2M | 125 | 60 |
| 39 | JongRES | €28.2M | 125 | 60 |
| 40 | nederlandse vereniging voor veterinair praktijk management | €26.3M | 125 | 60 |
| 41 | Rabobank Food & Agribusiness | €26.3M | 125 | 60 |
| 42 | XXImo | Leads the way in mobility | €26.3M | 125 | 60 |
| 43 | REMOBIS Refund Service C.V. | €26.3M | 125 | 60 |
| 44 | buckaroo | €26.3M | 125 | 60 |
| 45 | flexiq | €26.3M | 125 | 60 |
| 46 | pacer a deloitte business | €25.1M | 125 | 60 |
| 47 | Moovler | €24.8M | 125 | 60 |
| 48 | iter fidelis | €24.8M | 125 | 60 |
| 49 | Molhoek-CCT | €24.8M | 125 | 60 |
| 50 | fondo | €24.7M | 350 | 60 |
| 51 | Stichting Oranje Fonds | €23.8M | -- | 65 |
| 52 | de contentkalender | €23.1M | 125 | 60 |
| 53 | zigt (voorheen created first) | €23.1M | 125 | 60 |
| 54 | lfb landelijk | €23.1M | 125 | 60 |
| 55 | Hurenopstations.nl | €22.9M | 350 | 60 |
| 56 | 't sticht multi hygiene service b.v. | €21.8M | 350 | 60 |
| 57 | Techathone Systems BV | €21.8M | 125 | 60 |
| 58 | WCC | €21.3M | 125 | 60 |
| 59 | deXes | Building the Amsterdam Data Exchange | €21.3M | 125 | 60 |
| 60 | Landis Group B.V. | €21.3M | 125 | 60 |
| 61 | Landis ICT Group B.V. | €21.3M | 125 | 60 |
| 62 | BCN dé trainingslocaties | €20.5M | 125 | 60 |
| 63 | Gys Utrecht BV | €20.5M | 125 | 60 |
| 64 | brasserie & restaurant de rechtbank | €20.5M | 125 | 60 |
| 65 | BoulesBitesBar Utrecht | €20.3M | 125 | 60 |
| 66 | ZiN Kwaliteitsborging & Wabo | €18.9M | 125 | 60 |
| 67 | Koot Infrawerken | €18.9M | 125 | 60 |
| 68 | Utrechtse Bouw Societeit | €18.9M | 125 | 60 |
| 69 | STAN | Safe Trafficwork Application Network | €18.9M | 125 | 60 |
| 70 | Dura Vermeer Renovatie Midden West | €18.9M | 125 | 60 |
| 71 | conclusion low code company | €16.1M | 125 | 60 |
| 72 | Unusual Enterprise | €15.7M | 125 | 60 |
| 73 | Goodall Hoses - Industrial Hoses & Couplings | €15.3M | 125 | 60 |
| 74 | apac bedrijven groep | €15.3M | 125 | 60 |
| 75 | Van Rooijen Catering | €15.3M | 30 | 60 |
| 76 | Biology Masters Utrecht University | €14.3M | 125 | 60 |
| 77 | Onderwijskundig Leiderschap - HU Teaching & Learning Netwerk | €14.3M | 125 | 60 |
| 78 | stichting montessorischolen monton | €14.3M | 125 | 60 |
| 79 | Onderwijswetenschappen & ALPO | €14.3M | 125 | 60 |
| 80 | vakdidactiek geesteswetenschappen | €14.3M | 125 | 60 |
| 81 | NCB BV | €14.3M | 125 | 60 |
| 82 | Hogeschool TIO UTRECHT | €14.3M | 125 | 60 |
| 83 | Structure of Strength (SoS) | €14.3M | 125 | 60 |
| 84 | rzi- rijkelijk groen zaden international | €13.9M | 30 | 60 |
| 85 | hội anh street food | €13.9M | 30 | 60 |
| 86 | webacties.com | €13.8M | -- | 60 |
| 87 | vishandel volendam schilder (kil) b.v. | €13.8M | -- | 60 |
| 88 | loaf sourdough bakery | €13.8M | -- | 60 |
| 89 | stationsfoodstore | €13.8M | -- | 60 |
| 90 | sweet wonders | €13.8M | -- | 60 |
| 91 | alkautar | €13.8M | -- | 60 |
| 92 | shelter organics utrecht b.v. | €13.8M | -- | 60 |
| 93 | plus plantage | €13.8M | -- | 60 |
| 94 | suma supermarkten b.v. | €13.8M | -- | 60 |
| 95 | vishandel volendam overvecht | €13.8M | -- | 60 |
| 96 | candy print factory | €13.8M | -- | 60 |
| 97 | portale magico | €13.8M | -- | 60 |
| 98 | daymakers group | €13.7M | 125 | 60 |
| 99 | MOTORbeurs Utrecht | €13.7M | 125 | 60 |
| 100 | SurePay | €13.7M | 125 | 60 |