| 1 | Apollo Tyres (NL) B.V. | €914.5M | 9,900 | 70 |
| 2 | promocorp | €158.8M | 125 | 70 |
| 3 | het stedelijk lyceum enschede | €85.8M | 750 | 60 |
| 4 | PNO Consultants - Oost-Nederland | €73.6M | 350 | 60 |
| 5 | Schuurman Enschede Groothandel in Elektrotechniek | €51.5M | 125 | 60 |
| 6 | buttonboss group | €51.5M | 125 | 60 |
| 7 | Labora Quadratum | €51.0M | 125 | 60 |
| 8 | BSc and MSc (International) Business Administration UT | €40.1M | 350 | 60 |
| 9 | vco oost-nederland | €40.1M | 350 | 60 |
| 10 | Renault Munsterhuis | €38.1M | 125 | 60 |
| 11 | sign top bv | €38.1M | 30 | 60 |
| 12 | tzc - twentsche zweefvlieg club | €34.6M | 125 | 60 |
| 13 | HESS AAC SYSTEMS | €29.2M | 125 | 60 |
| 14 | TechNet Oost | €25.0M | 125 | 60 |
| 15 | MECAL Independent eXperts | €25.0M | 125 | 60 |
| 16 | Delwi/Groenink Machinefabriek | €25.0M | 125 | 60 |
| 17 | auto verkopen ikwilvanmijnautoaf.nl | €23.8M | 125 | 60 |
| 18 | Peterman Lease, uw partner voor elk merk | €23.8M | 125 | 60 |
| 19 | D-NL Business-House | €23.0M | 30 | 60 |
| 20 | Brookhuis Applied Data Intelligence b.v. | €23.0M | 30 | 60 |
| 21 | ice-africa (also trading as african energy & consulting) | €23.0M | 30 | 60 |
| 22 | sesam bv | €22.5M | 125 | 60 |
| 23 | Onderhoud Enschede bv | €21.8M | 350 | 60 |
| 24 | dbc re-integratie bv | revalidatie bv | €20.9M | 125 | 60 |
| 25 | kooy insulation b.v. | €18.9M | 125 | 60 |
| 26 | Raam Wereld BV | €18.9M | 125 | 60 |
| 27 | School of Regional Healthcare Transformation (SoRHT) | €18.9M | 350 | 60 |
| 28 | s.v. tuks | €16.1M | 125 | 60 |
| 29 | Faculty of Geo-Information Science and Earth Observation (ITC) of the University of Twente | €14.3M | 125 | 60 |
| 30 | ITC | Applied Earth Sciences (AES) department | UTwente (NL) | €14.3M | 125 | 60 |
| 31 | ROC van Twente Opleiding Medewerker Business Support | €14.3M | 125 | 60 |
| 32 | grolsch international b.v. | €13.9M | 30 | 60 |
| 33 | spar city enschede | €13.8M | -- | 60 |
| 34 | meerdanvlees.nl | €13.8M | -- | 60 |
| 35 | supermarkt ada import export | €13.8M | -- | 60 |
| 36 | bhodey boys coffee club | €13.8M | -- | 60 |
| 37 | sanders supermarkten | €13.8M | -- | 60 |
| 38 | Fc de Rebellen Entertainment | €13.7M | 125 | 60 |
| 39 | Maas v.'t Hoog Zeefdr. en Rekl.-Industr. B.V. | €13.0M | 125 | 60 |
| 40 | centre for disaster resilience | €12.6M | 125 | 60 |
| 41 | ZB Service BV | €12.4M | 30 | 60 |
| 42 | Landewé Bike Totaal | €12.2M | 30 | 60 |
| 43 | Motection | €12.2M | 30 | 60 |
| 44 | Hollandia Matzes B.V. | €12.2M | 30 | 60 |
| 45 | Van der Moolen Foodgroup bv | €12.2M | 30 | 60 |
| 46 | Sigmax Field Service Solutions | €11.8M | 125 | 60 |
| 47 | profi kozijnen b.v. | €11.8M | 30 | 60 |
| 48 | ReadID by Inverid | €11.1M | 125 | 60 |
| 49 | zorggroep manna | €9.4M | 750 | 50 |
| 50 | ql-ict | €8.8M | 125 | 50 |
| 51 | atak | €8.3M | 125 | 50 |
| 52 | Tubantia Economie | €8.3M | 125 | 50 |
| 53 | Brünen Bedrijfsopleidingen | €8.2M | 30 | 50 |
| 54 | willemsen schoonmaakorganisatie b.v. | €7.8M | 125 | 50 |
| 55 | Machnet Medical Robotics | €7.3M | 30 | 50 |
| 56 | Omega Engineering B.V. | €7.0M | 30 | 50 |
| 57 | MECAL Wind Turbine Design B.V. | €7.0M | 30 | 50 |
| 58 | MECAL Wind / Advanced Tower Systems | €6.8M | 30 | 50 |
| 59 | gelria pakking bv | €6.8M | 30 | 50 |
| 60 | hug engineering b.v. | €6.8M | 30 | 50 |
| 61 | SMT Zorg & Zorgadvies | €6.7M | 125 | 50 |
| 62 | KienhuisHoving advocaten en notarissen | €6.3M | 125 | 50 |
| 63 | kienhuishoving n.v. | €6.3M | 125 | 50 |
| 64 | Bouw Consulting Holding B.V. | €6.3M | 30 | 50 |
| 65 | Esschert Design Vastgoed B.V. | €6.3M | 30 | 50 |
| 66 | Sanders Supermarkten B.V. | €6.3M | 30 | 50 |
| 67 | Green Team Twente | €5.9M | 30 | 50 |
| 68 | looping.nl | €5.9M | 30 | 50 |
| 69 | ABS Dick Bösing | €5.9M | 30 | 50 |
| 70 | Kolenaar Mobiliteit - Mazda | €5.9M | 30 | 50 |
| 71 | CraTeX | €5.9M | 30 | 50 |
| 72 | Ontstoppen Enschede Riool, Afvoer, Wc & Gootsteen | €5.9M | 30 | 50 |
| 73 | Indulux Led professionals | €5.7M | 30 | 50 |
| 74 | de wolf businessclub | €5.5M | 30 | 50 |
| 75 | Branding by Banding | €5.5M | 30 | 50 |
| 76 | Ipskamp Printing - Thesis and more | €5.5M | 30 | 50 |
| 77 | Spock | €5.1M | 30 | 50 |
| 78 | thereseller.store | €5.1M | 30 | 50 |
| 79 | tjecco® - GebouwCompliance software | €5.1M | 30 | 50 |
| 80 | Score Media B.V. | €5.1M | 30 | 50 |
| 81 | Appnormal | €5.1M | 30 | 50 |
| 82 | Fumico Fumigations B.V. | €5.0M | 30 | 50 |
| 83 | nightclub collective enschede (nice) | €4.9M | 30 | 50 |
| 84 | aquaquick global bv | €4.9M | 30 | 50 |
| 85 | break-even | €4.9M | 30 | 50 |
| 86 | Pro-F Fysiotherapie | €4.9M | 30 | 50 |
| 87 | body-perfection | €4.9M | 30 | 50 |
| 88 | Finovion Enschede | €4.6M | 125 | 50 |
| 89 | Veilig Vakwerk | €4.5M | 30 | 50 |
| 90 | Dirk Nijhuis en Appels Schilderwerken BV (DN&A) | €4.5M | 30 | 50 |
| 91 | ZonEx Binnen- & Buitenleven | €4.5M | 30 | 50 |
| 92 | Bouwbedrijf H.J. Assink Enschede B.V. | €4.5M | 30 | 50 |
| 93 | drukkerij veltmann b.v. | €3.9M | 30 | 50 |
| 94 | OSC Overseas Consultancy | €3.8M | 5 | 50 |
| 95 | New Enschede Technologies B.V. | €3.8M | 5 | 50 |
| 96 | PCE Brookhuis BV | €3.8M | 5 | 50 |
| 97 | Fast F#cking Forward | €3.8M | 30 | 50 |
| 98 | Beckmann Groep B.V. | €3.7M | 30 | 50 |
| 99 | finapres medical systems | €3.7M | 30 | 50 |
| 100 | Speakup | pioneering in communication | €3.5M | 30 | 50 |