| 1 | PHOENIX Pharmahandel GmbH & Co KG | €28.2B | -- | 69 |
| 2 | Südzucker AG | €9.7B | -- | 69 |
| 3 | röchling automotive se & co. kg | €1.4B | 7,500 | 80 |
| 4 | Pepperl+Fuchs SE | €369.7M | 372 | 69 |
| 5 | DRK -Blutspendedienst Baden-Württemberg-Hessen gemeinnützige GmbH | €166.2M | 1 | 69 |
| 6 | Etengo AG | €132.7M | 198 | 69 |
| 7 | MVV Enamic GmbH | €96.0M | 350 | 60 |
| 8 | BridgingIT GmbH | €86.8M | 35 | 69 |
| 9 | Roche Diabetes Care Deutschland | €85.6M | 350 | 60 |
| 10 | Sax + Klee GmbH Bauunternehmung | €85.2M | 2,021 | 69 |
| 11 | Röchling Medical SE | €77.1M | 2,020 | 69 |
| 12 | Deutsche Rohstoff AG | €73.3M | 26 | 69 |
| 13 | DOPAG – Metering and Mixing Technology | €70.0M | 350 | 60 |
| 14 | e+p+i europe GmbH | €64.4M | 125 | 60 |
| 15 | Röchling Automotive SE | €63.5M | 4 | 69 |
| 16 | Reputation.com (DACH) | €59.6M | 350 | 60 |
| 17 | SZA Schilling, Zutt & Anschütz Rechtsanwaltsgesellschaft mbH | €54.3M | 36 | 69 |
| 18 | DSL Schweisstechnik GmbH | €51.5M | 125 | 60 |
| 19 | H. Ristelhueber's Nachfolger Spedition GmbH | €51.0M | 125 | 60 |
| 20 | ad-AUTOTEILE-CARGO GmbH & Co. KG | €51.0M | 125 | 60 |
| 21 | KYOCERA Fineceramics Europe GmbH | €48.4M | 2,022 | 69 |
| 22 | ALMAS INDUSTRIES AG | €40.3M | 350 | 60 |
| 23 | drivemotive | €38.1M | 125 | 60 |
| 24 | e-47 GmbH | €35.6M | -- | 69 |
| 25 | Comfort Hotel Beteiligungs GmbH | €33.3M | 2 | 69 |
| 26 | MOVILIZER GMBH | €30.6M | -- | 69 |
| 27 | RAD-x Management GmbH | €26.8M | 700 | 69 |
| 28 | management consult Dr. Eisele & Dr. Noll GmbH | €23.1M | 125 | 60 |
| 29 | häuselmann metall GmbH | €22.4M | -- | 69 |
| 30 | si electronics | €21.7M | 125 | 60 |
| 31 | Rapinno Tech Germany | €21.3M | 125 | 60 |
| 32 | gospel music | €20.4M | 3,000 | 60 |
| 33 | Rütgers GmbH & Co. KG Kälte Klima | €18.9M | 125 | 60 |
| 34 | Precasta | €18.9M | 125 | 60 |
| 35 | FANTASY PHARMA | €18.0M | 125 | 60 |
| 36 | Jonastone Natursteine | €17.8M | 30 | 60 |
| 37 | ELSA-Mannheim e. V. | €17.7M | 350 | 60 |
| 38 | Rocket Software Deutschland GmbH | €16.0M | -- | 69 |
| 39 | Rocket Software Technologies Deutschland GmbH & Co. KG | €16.0M | -- | 69 |
| 40 | Fachschaftsinitiative Medizin Mannheim e.V. | €14.3M | 125 | 60 |
| 41 | Scope International AG | €14.0M | 248 | 69 |
| 42 | AstraDirect SCHLIESSFÄCHER GmbH | €14.0M | 33 | 69 |
| 43 | Financial Services GmbH | €13.8M | 8,000 | 69 |
| 44 | PAUL Tech AG | €13.3M | -- | 69 |
| 45 | Volksbank Sandhofen eG | €12.3M | 49 | 69 |
| 46 | Loady - making logistics easier | €12.2M | 30 | 60 |
| 47 | QI Post | €12.2M | 30 | 60 |
| 48 | Power Plus Communications AG | €12.2M | 89 | 69 |
| 49 | socomec deutschland | €12.2M | 125 | 60 |
| 50 | Mannheim Business School gGmbH | €12.2M | 79 | 69 |
| 51 | INTE Informationstechnologie GmbH | €11.6M | 87 | 69 |
| 52 | Netzwerk Smart Production | €10.8M | 30 | 60 |
| 53 | o+p consult gmbh | €10.6M | 30 | 60 |
| 54 | sentronics metrology GmbH | €8.6M | 27 | 69 |
| 55 | GE POWER GMBH | €8.5M | -- | 69 |
| 56 | Steuertipps.de | €8.5M | 125 | 50 |
| 57 | DLC Mannheim | €8.2M | 30 | 50 |
| 58 | H. J. Waldkirch Taxibetrieb | €8.2M | 30 | 50 |
| 59 | Stadtpark Mannheim gemeinnützige GmbH | €8.2M | 2,020 | 69 |
| 60 | 1stQ Deutschland GmbH | €7.3M | 30 | 50 |
| 61 | Earth Guardians Mannheim | €6.8M | 30 | 50 |
| 62 | VAG Holding GmbH | €6.6M | -- | 69 |
| 63 | JÖRGER Armaturen- und Accessoires-Fabrik GmbH | €6.6M | 30 | 50 |
| 64 | Stadler & Partner | €6.3M | 30 | 50 |
| 65 | Mannheim Invest Tours | €6.3M | 30 | 50 |
| 66 | AK Advisory by AKB | €6.3M | 30 | 50 |
| 67 | Erhard Kühn GmbH | €5.6M | 24 | 69 |
| 68 | r-m-p architekten und ingenieure | €5.5M | 30 | 50 |
| 69 | CloudRail | €5.1M | 30 | 50 |
| 70 | CLS Soft | €5.1M | 30 | 50 |
| 71 | astradirect Schließfächer GmbH | €5.0M | 30 | 50 |
| 72 | mannheim supply chain students | €4.9M | 30 | 50 |
| 73 | STAYTION Hotel & Co-Working | €4.9M | 30 | 50 |
| 74 | Private University Europa Campus | €4.1M | 350 | 50 |
| 75 | VaPassion | €4.1M | 125 | 50 |
| 76 | Theodor-Fliedner-Stiftung | €3.9M | 78 | 69 |
| 77 | flyweight | €3.8M | 5 | 50 |
| 78 | kuehlhaus AG - Digital Experience Agency | €3.8M | 125 | 50 |
| 79 | UX-DAY | €3.8M | 125 | 50 |
| 80 | flexword® translators & consultants | €3.8M | 30 | 50 |
| 81 | inno.space - Design Factory Mannheim | €3.4M | 30 | 50 |
| 82 | Corps Hermunduria Leipzig | €3.4M | 30 | 50 |
| 83 | University of Applied Management Studies | €3.4M | 30 | 50 |
| 84 | Alanus Hochschule - Institut für Waldorfpädagogik | €3.4M | 30 | 50 |
| 85 | myTalkmaster.de | €3.4M | 30 | 50 |
| 86 | TCI Transformation Consulting International | €3.4M | 125 | 50 |
| 87 | emv gmbh - medien- veranstaltungstechnik | €3.3M | 30 | 50 |
| 88 | Fleck's Factory | €3.3M | 30 | 50 |
| 89 | HRM Institute GmbH & Co KG | €3.3M | 30 | 50 |
| 90 | customysocks.com | €3.1M | 30 | 50 |
| 91 | omer karakus interior design and architecture | €3.1M | 30 | 50 |
| 92 | Parkhausbetriebe Mannheim | €3.0M | 30 | 50 |
| 93 | adago | €2.8M | 30 | 50 |
| 94 | Univere UG (haftungsbeschränkt) | €2.8M | 30 | 50 |
| 95 | ooblee | €2.8M | 30 | 50 |
| 96 | CAIRO AG, IT & Software | €2.8M | 30 | 50 |
| 97 | Sally Sales Assistant | €2.8M | 30 | 50 |
| 98 | Cybergroup International - Promotional items Fullservice, Merchandise, Fulfillment Logistics | €2.7M | 125 | 50 |
| 99 | The Hackathon Company | €2.7M | 30 | 50 |
| 100 | marmalade Group GmbH | €2.7M | 30 | 50 |