| 1 | TAKEDA GMBH | €130.3M | 210 | 69 |
| 2 | Motan Holding GmbH | €116.2M | -- | 69 |
| 3 | Ingun Prüfmittelbau GmbH | €73.9M | -- | 69 |
| 4 | Bracco Imaging Deutschland GmbH | €53.7M | 43 | 69 |
| 5 | Schwarz-Außenwerbung GmbH | €41.8M | 66 | 69 |
| 6 | HTWG Hochschule Konstanz – Technik, Wirtschaft und Gestaltung | €40.1M | 350 | 60 |
| 7 | monteiros | metalworking industry | €32.4M | 125 | 60 |
| 8 | SEITENBAU GmbH | €23.2M | 187 | 69 |
| 9 | cobra computer's brainware GmbH | €21.3M | 125 | 60 |
| 10 | Playlife Sports | €20.6M | 125 | 60 |
| 11 | farmtiger | €19.7M | 30 | 60 |
| 12 | Mainau GmbH | €17.2M | 244 | 69 |
| 13 | snackonaut | €13.8M | -- | 60 |
| 14 | Caritas-Altenhilfe für die Region Konstanz gemeinnützige GmbH | €12.7M | 303 | 69 |
| 15 | RIVAFY | €12.2M | 30 | 60 |
| 16 | ABACON GmbH | €11.8M | 125 | 60 |
| 17 | Spar- und Bauverein Konstanz eG | €8.9M | 2,353 | 69 |
| 18 | Carlack Gesellschaft für Technik und Handel mbH | €8.7M | 30 | 50 |
| 19 | Maptoolkit | €5.1M | 30 | 50 |
| 20 | südkurier citylogistik | €4.9M | 30 | 50 |
| 21 | Betonkanu HTWG Konstanz | €4.5M | 30 | 50 |
| 22 | Kleiner Fuchs | €3.8M | 5 | 50 |
| 23 | Phi Delta Phi - Udo Di Fabio-Inn Konstanz e.V. | €3.4M | 30 | 50 |
| 24 | Allensbach Hochschule Konstanz | €3.4M | 30 | 50 |
| 25 | Zentrum für Mehrsprachigkeit Konstanz | €3.4M | 30 | 50 |
| 26 | kilometer1 | €3.4M | 30 | 50 |
| 27 | Startuplounge Bodensee | €3.3M | 30 | 50 |
| 28 | T–8.space | €3.1M | 30 | 50 |
| 29 | IST Innovationsinstitut | €3.1M | 30 | 50 |
| 30 | MIK GmbH - a Unit4 company | €2.7M | 30 | 50 |
| 31 | timeghost GmbH | €2.7M | 30 | 50 |
| 32 | Xanevo - Text Generation for Fashion eCommerce | €2.7M | 30 | 50 |
| 33 | LA PASSION * bridal and evening wear | €2.0M | 5 | 50 |
| 34 | Nordkit | €2.0M | 5 | 50 |
| 35 | Jester-Sports | €2.0M | 5 | 50 |
| 36 | bitco³ | €1.8M | 5 | 50 |
| 37 | Center for Alternatives to Animal Testing (CAAT) | €1.6M | 30 | 50 |
| 38 | tpa GmbH - HR Services | €1.5M | 30 | 50 |
| 39 | garten am seerhein | €1.4M | 5 | 50 |
| 40 | N° elf Spezialitätenkaffee Konstanz | €1.2M | 5 | 50 |
| 41 | Metallization & Interconnection Workshop | €1.1M | 5 | 50 |
| 42 | H2C Group | €1.1M | 5 | 50 |
| 43 | 22-21 | twenty-two twenty-one jan pütz | €1.1M | 5 | 50 |
| 44 | Fallout Film Medien Filmproduktion DVD Imagefilm Lehrfilme | €1.1M | 5 | 50 |
| 45 | krehl.girke architekten | €1.1M | 30 | 50 |
| 46 | WikiHouse.studies+ | €1.1M | 30 | 50 |
| 47 | Ebner Ingenieurbüro | €1.0M | 5 | 40 |
| 48 | Braun + Müller Architekten BDA ● Wettbewerbe | €1.0M | 5 | 40 |
| 49 | Martin Krehl & Rainer Girke krehl.girke architekten | €1.0M | 5 | 40 |
| 50 | Elektronik & Steuerungstechnik Pfeifer Adam Domin e.K. | €950K | 5 | 40 |
| 51 | nimmerland theaterproduktion | €929K | 5 | 40 |
| 52 | lehmbau.büro | €925K | 5 | 40 |
| 53 | 99needs gmbh | €923K | 5 | 40 |
| 54 | movawo | €923K | 5 | 40 |
| 55 | Vhite Rabbit | €851K | 5 | 40 |
| 56 | TimeScout - Home of the Activity-Report | €851K | 5 | 40 |
| 57 | ECOCAMPING Service GmbH | €813K | 30 | 40 |
| 58 | Lorth Gessler Mittelstaedt GmbH | €649K | 30 | 40 |
| 59 | konstech | €644K | 5 | 40 |
| 60 | validateml gmbh | €644K | 5 | 40 |
| 61 | ihk industrie und handelskammer konstanz | €644K | 5 | 40 |
| 62 | Brand Development ▫️ Brand Design ▫️ Brand Experience ▫️ Kommunikationsmedien ▫️ Film | €627K | 5 | 40 |
| 63 | Valtrado - translation and text services | €627K | 5 | 40 |
| 64 | sgiarovello design | €627K | 5 | 40 |
| 65 | Technische Akademie Konstanz - Institut für wissenschaftliche Weiterbildung an der HTWG Konstanz | €572K | 5 | 40 |
| 66 | habitivity gmbh | €572K | 5 | 40 |
| 67 | Exzellenzcluster "Kulturelle Grundlagen von Integration" | €572K | 5 | 40 |
| 68 | BODENSEE SUMMIT digital | €548K | 5 | 40 |
| 69 | Laib Yala Tricot | €519K | 5 | 40 |
| 70 | Touch of Alps | €495K | 5 | 40 |
| 71 | 8-BOT | €473K | 5 | 40 |
| 72 | AI4Future Dataconsulting Philipp Scharpf | €473K | 5 | 40 |
| 73 | trink' das! | €465K | 5 | 40 |
| 74 | EU Careers Konstanz | €429K | 5 | 40 |
| 75 | eonamic - Filmproduktion & Drohnenaufnahmen | €331K | 5 | 40 |
| 76 | Elevisto | €328K | 5 | 40 |
| 77 | TECTUM Real Property | €328K | 5 | 40 |
| 78 | Kutter Law | Erbrecht und Steuerrecht | €253K | 5 | 40 |
| 79 | steg2drittel - Agentur für Konfliktmanagement | €165K | 5 | 40 |
| 80 | icegorilla | digital gedöns | €151K | 5 | 40 |
| 81 | thepalia | digitale praxisplattform | €141K | 5 | 40 |
| 82 | Heerschlag Web-Entwickler | €136K | 5 | 40 |
| 83 | MY BEST LIFE GbR | €136K | 5 | 40 |
| 84 | Stefan Engels Agile Coaching & Consulting | €136K | 5 | 40 |
| 85 | mein persönliches konzept gmbh | €136K | 5 | 40 |
| 86 | Caritas Konstanz GmbH | €123K | 5 | 40 |
| 87 | Rockntrail | €115K | 5 | 40 |
| 88 | ELVL - Level up your game! | €115K | 5 | 40 |
| 89 | BNI Region Konstanz | €108K | 5 | 40 |
| 90 | LeadMedics - Medical Marketing | €108K | 5 | 40 |
| 91 | samsmedia | Social Media & Webdesign | €108K | 5 | 40 |
| 92 | Virtuelle Assistenz Nadine Kelm | €108K | 5 | 40 |
| 93 | Lovely Giants – Videoproduktion | €108K | 5 | 40 |
| 94 | Bodenseeblog Konstanz Leben und genießen | €108K | 5 | 40 |
| 95 | brandb - Employer Branding, Personalmarketing, Social Recruiting | €108K | 5 | 40 |
| 96 | Sucks Socks | €105K | 5 | 40 |
| 97 | mor khadi | €105K | 5 | 40 |
| 98 | Lake Constance Arts & Sciences Association | €59K | 5 | 30 |
| 99 | DACHmasters | €59K | 5 | 30 |
| 100 | avanto 3 - die unternehmensentwickler | €53K | 5 | 30 |