| 1 | Heidelberg Materials AG | €17.6B | 53,122 | 69 |
| 2 | SRH Holding | €1.3B | 15,304 | 69 |
| 3 | Deutsches Krebsforschungszentrum | €319.0M | -- | 69 |
| 4 | Chemische Werke Kluthe GmbH | €176.0M | 132 | 69 |
| 5 | ProMinent GmbH | €144.6M | 79 | 69 |
| 6 | Questax AG | €99.1M | 388 | 69 |
| 7 | Heidelberg Engineering GmbH | €94.2M | 70 | 69 |
| 8 | BASF Forward AM | €80.1M | 350 | 60 |
| 9 | accenture software for human capital management | €52.9M | 750 | 60 |
| 10 | eBuch Unternehmensgruppe | €51.5M | 125 | 60 |
| 11 | Rhein-Neckar-Zeitung Gesellschaft mit beschränkter Haftung | €48.7M | 2 | 69 |
| 12 | GetSafe - Personal Insurance Manager | €45.9M | 125 | 60 |
| 13 | AM International GmbH | €44.8M | 218 | 69 |
| 14 | E-Learning und Medienbildung | €40.1M | 350 | 60 |
| 15 | io-consultants GmbH & Co. KG | €36.1M | 283 | 69 |
| 16 | Solar PV 10 GmbH & Co. KG | €35.6M | -- | 69 |
| 17 | IssaMiningPro | €33.3M | 125 | 60 |
| 18 | SRH Berufliche Rehabilitation GmbH | €28.7M | 454 | 69 |
| 19 | PLASTVERARBEITER | €28.6M | 125 | 60 |
| 20 | Evangelische Stiftung Pflege Schönau | €28.0M | -- | 69 |
| 21 | Automated Precision Europe GmbH | €25.0M | 125 | 60 |
| 22 | Volume Graphics, part of Hexagon | €21.3M | 125 | 60 |
| 23 | Geuder Aktiengesellschaft | €19.7M | 76 | 69 |
| 24 | National Center for Tumor Diseases (NCT) Heidelberg | €18.9M | 350 | 60 |
| 25 | Pro Bono Heidelberg e.V. | €17.7M | 350 | 60 |
| 26 | Institute for Translation and Interpreting - University of Heidelberg | €15.7M | 125 | 60 |
| 27 | HSAG Heidelberger Services AG | €15.0M | -- | 69 |
| 28 | Breer Gebäudedienste Heidelberg GmbH | €14.7M | 694 | 69 |
| 29 | Gustav Adolf GmbH | €14.6M | 26 | 69 |
| 30 | International Master of Science in Social Protection | €14.3M | 125 | 60 |
| 31 | 4EU+ Alliance | €14.3M | 125 | 60 |
| 32 | Onapsis Europe GmbH | €14.2M | 811 | 69 |
| 33 | sovanta AG | €14.1M | 150 | 69 |
| 34 | halle02 & Güterbahnhof Heidelberg | €13.7M | 125 | 60 |
| 35 | HMS Analytical Software GmbH | €13.1M | 100 | 69 |
| 36 | edelweiss GmbH | €12.1M | -- | 69 |
| 37 | Winkler AG | €12.1M | 200 | 69 |
| 38 | Heidelberger Unternehmerinnen e.V. | €11.9M | 125 | 60 |
| 39 | HMS Analytical Software | Consulting & End-to-End Solutions for Data Science & Analytics | €11.8M | 125 | 60 |
| 40 | Vereinigte Baustoff- und Eisen-GmbH | €11.8M | 54 | 69 |
| 41 | PromoCell GmbH | €11.7M | 85 | 69 |
| 42 | Mensch-Sein mit Algorithmen / Being Human with Algorithms | €11.1M | 125 | 60 |
| 43 | JKB GmbH | €11.0M | -- | 69 |
| 44 | «Академия отрасли профессиональных услуг» (Академия APSI) | €9.5M | 350 | 50 |
| 45 | Heidelberger Straßen- und Bergbahn GmbH | €9.2M | -- | 69 |
| 46 | nature2need I Biopolymer & Biomaterial Compounds I Circular Economy | €8.7M | 30 | 50 |
| 47 | Gubener Plastinate GmbH | €7.9M | 86 | 69 |
| 48 | ERNW Enno Rey Netzwerke GmbH | €7.7M | -- | 69 |
| 49 | heidelberger payment gmbh | €7.5M | 125 | 50 |
| 50 | Neurochirurgische Klinik Universitätsklinikum Heidelberg | €6.7M | 125 | 50 |
| 51 | Heidelberger Vermögen | €6.3M | 30 | 50 |
| 52 | Questax Experts GmbH | €5.5M | 25 | 69 |
| 53 | Quantfloor GmbH & Co. KG | €5.2M | 30 | 50 |
| 54 | Nemlia | €5.1M | 30 | 50 |
| 55 | Astriol Digital GmbH | €5.1M | 30 | 50 |
| 56 | igst - Internationale Gesellschaft für Systemische Therapie e. V. | €5.0M | 30 | 50 |
| 57 | Getsafe Digital GmbH | €5.0M | -- | 69 |
| 58 | Getsafe GmbH | €5.0M | -- | 69 |
| 59 | Andrzej Wisniewski | €4.5M | 30 | 50 |
| 60 | istitute of public health - university of heidelberg | €4.4M | 350 | 50 |
| 61 | ENMON GmbH | €4.1M | 8 | 69 |
| 62 | EMBL Enterprise Management Technology Transfer GmbH | €4.1M | -- | 69 |
| 63 | Curagita Holding GmbH | €3.9M | 1 | 69 |
| 64 | goldn | €3.9M | 30 | 50 |
| 65 | cocape Regenschutz | €3.8M | 5 | 50 |
| 66 | cocape fahrrad-regenschutz | €3.8M | 5 | 50 |
| 67 | Drupal e. V. | €3.8M | 125 | 50 |
| 68 | io-consultants | €3.7M | 350 | 50 |
| 69 | everest systems inc | €3.5M | 125 | 50 |
| 70 | rn sicherheit gmbh | €3.5M | 30 | 50 |
| 71 | ACQUIFER Imaging | €3.1M | 30 | 50 |
| 72 | IMSEAM - Selhuber Unkel Group | €3.1M | 30 | 50 |
| 73 | Neuromorphe Quantenphotonik | €3.0M | 30 | 50 |
| 74 | Mediteo | €2.8M | 30 | 50 |
| 75 | HexaMain | €2.8M | 30 | 50 |
| 76 | Leanbyte - Manufacturing Qualification System | €2.8M | 30 | 50 |
| 77 | Medical Talent DE | €2.6M | 30 | 50 |
| 78 | conciervis concierge service & app! | €2.1M | 30 | 50 |
| 79 | ibv wohnungsunternehmen | €2.0M | 30 | 50 |
| 80 | Europaplatz Heidelberg | €2.0M | 30 | 50 |
| 81 | saferadon gmbh | €2.0M | 5 | 50 |
| 82 | 1a-Gebäudereinigung Pforzheim | €1.9M | 30 | 50 |
| 83 | finous llc | €1.8M | 5 | 50 |
| 84 | Shift Medical | €1.6M | 30 | 50 |
| 85 | AK Digitale Medizin Heidelberg | €1.6M | 30 | 50 |
| 86 | BALAJI GmbH | €1.5M | 30 | 50 |
| 87 | HR-Meter | €1.5M | 30 | 50 |
| 88 | nextx | €1.5M | 30 | 50 |
| 89 | BPP - Better Peptides | €1.4M | 5 | 50 |
| 90 | heidelberg-tourguide.de | €1.4M | 5 | 50 |
| 91 | AMERIA AG | €1.3M | -- | 69 |
| 92 | Rudolf Entenmann GmbH | €1.3M | 5 | 50 |
| 93 | Eliya Tea Project | €1.2M | 5 | 50 |
| 94 | Maxcert Germany | €1.2M | 5 | 50 |
| 95 | TJ Medical Advise | €1.2M | 5 | 50 |
| 96 | movitale a brand of PDIG | €1.2M | 5 | 50 |
| 97 | BlueActivity | €1.1M | 5 | 50 |
| 98 | HR-FuelCell | €1.1M | 5 | 50 |
| 99 | symovo gmbh | €1.1M | 5 | 50 |
| 100 | Wirtschaftsjunioren Heidelberg e.V. | €1.1M | 125 | 50 |