| 1 | E. Merck KG | €7.1B | 3,601 | 69 |
| 2 | merck surface solutions | €3.3B | 20,000 | 80 |
| 3 | AERO INVEST GmbH | €294.7M | 585 | 69 |
| 4 | PROFI Engineering Systems AG | €156.8M | 2,022 | 69 |
| 5 | Mission Leben gGmbH | €113.0M | 1,554 | 69 |
| 6 | e-netz-südhessen | €96.2M | 750 | 60 |
| 7 | Varian Medical Systems Deutschland GmbH & Co. KG | €92.3M | -- | 69 |
| 8 | KBS Group GmbH | €64.5M | 750 | 69 |
| 9 | Telekom Voicification Suite | €59.6M | 350 | 60 |
| 10 | EAB Elektroanlagenbau GmbH Rhein/Main Niederlassung Darmstadt | €52.9M | 350 | 60 |
| 11 | Software AG - Stiftung | €51.2M | -- | 69 |
| 12 | maila-push GmbH | €45.1M | 350 | 60 |
| 13 | ab enzymes | €36.4M | 350 | 60 |
| 14 | CultArm3D | €33.1M | 350 | 60 |
| 15 | VDD - Verein Deutscher Druckingenieure e.V. | €22.9M | 125 | 60 |
| 16 | [ui!] | €21.3M | 125 | 60 |
| 17 | Patagona GmbH | €21.3M | 125 | 60 |
| 18 | wohnpark kranichstein gmbh | €20.9M | 125 | 60 |
| 19 | Accso - Accelerated Solutions GmbH | €18.3M | 132 | 69 |
| 20 | Apotheken-Rechen-Zentrum Gesellschaft mit beschränkter Haftung. | €17.1M | 186 | 69 |
| 21 | Mission Leben - Jugend- und Behindertenhilfe gGmbH | €14.9M | -- | 69 |
| 22 | Corps Obotritia Darmstadt | €14.3M | 125 | 60 |
| 23 | Evangelische Erwachsenenbildung EKHN | €14.3M | 125 | 60 |
| 24 | Martin-Behaim-Schule Darmstadt | €14.3M | 125 | 60 |
| 25 | Corps Franconia Darmstadt | €14.3M | 125 | 60 |
| 26 | Corps Chattia Darmstadt | €14.3M | 125 | 60 |
| 27 | Darmstadt Center for Logistics | €12.2M | 30 | 60 |
| 28 | B&B 24 Stiftung | €11.0M | 18,474 | 69 |
| 29 | cst - computer simulation technology | €9.9M | 350 | 50 |
| 30 | Liquid Crystal Windows by EMD Performance Materials | €8.7M | 30 | 50 |
| 31 | Bürostuhl Shop | €7.2M | 30 | 50 |
| 32 | KBS GmbH & Co. KG | €7.0M | 54 | 69 |
| 33 | Creditreform Skoruppa KG | €6.3M | 30 | 50 |
| 34 | Fördersysteme Engeneering GmbH | €6.0M | 30 | 50 |
| 35 | Kfz-Werkstatt J. Heiden GmbH in Darmstadt | €5.9M | 30 | 50 |
| 36 | [email protected] | €5.2M | 30 | 50 |
| 37 | Empolis Intelligent Views GmbH | €5.1M | 30 | 50 |
| 38 | MFS - Moldflow, Alias & VRED | €5.1M | 30 | 50 |
| 39 | RapidCompact by DGG | €5.1M | 30 | 50 |
| 40 | healthy business consulting | €5.0M | 30 | 50 |
| 41 | Hotel Jagdschloss Kranichstein GmbH | €4.9M | 30 | 50 |
| 42 | Ludendorff Niederlassung | €4.5M | 30 | 50 |
| 43 | planquadrat Elfers Geskes Krämer und Partner GmbH | €4.5M | 125 | 50 |
| 44 | KTBL | €3.8M | 125 | 50 |
| 45 | Multimedia Communications Lab (KOM), TU Darmstadt | €3.4M | 30 | 50 |
| 46 | hessen-technikum | €3.4M | 30 | 50 |
| 47 | SEAC - Space Economy Academy | €3.4M | 30 | 50 |
| 48 | Reactive Flows and Diagnostics, TU Darmstadt | €3.4M | 30 | 50 |
| 49 | Unternehmer*innenteam Darmstadt BNI Lichtenberg | €3.1M | 30 | 50 |
| 50 | justizvollzugsanstalt darmstadt fritz-bauer-haus | €3.0M | 30 | 50 |
| 51 | entplexit GmbH | €2.8M | 30 | 50 |
| 52 | etalytics GmbH | €2.8M | 30 | 50 |
| 53 | Cloud Consulting Europe (CCE) | €2.8M | 30 | 50 |
| 54 | ConWeaver GmbH | €2.8M | 30 | 50 |
| 55 | CircuitByte | €2.8M | 30 | 50 |
| 56 | nubit. GmbH | €2.7M | 30 | 50 |
| 57 | BidX - Master Amazon Advertising | €2.7M | 30 | 50 |
| 58 | Dr. Terhalle Personalberatung GmbH | €2.6M | 30 | 50 |
| 59 | itl gmbh - software to reach your aim | €2.1M | 30 | 50 |
| 60 | DIAMANTEN EXPRESS | €2.1M | 5 | 50 |
| 61 | Frankenstein Bikes | €2.0M | 5 | 50 |
| 62 | Fein & Rein Gebäudeservice GmbH & Co. KG | €1.9M | 30 | 50 |
| 63 | ARAG Versicherung Matthias Klein | €1.8M | 5 | 50 |
| 64 | steuerberatung hofmann & partner | €1.8M | 30 | 50 |
| 65 | kraus system support | €1.8M | 5 | 50 |
| 66 | profit100 consulting | €1.8M | 5 | 50 |
| 67 | DENIC Services GmbH & Co. KG | €1.5M | 1 | 69 |
| 68 | Iffland Wischnewski Rechtsanwälte | €1.5M | 30 | 50 |
| 69 | OK Rechtsanwälte | €1.5M | 30 | 50 |
| 70 | Rechtsanwälte u. Notar Dr. h.c. Lankau, Dr. Weitz & Collegen PartGmbB | €1.5M | 30 | 50 |
| 71 | Badehaus Film | €1.5M | 5 | 50 |
| 72 | Darmstadt Business School | Advanced Education | €1.5M | 125 | 50 |
| 73 | cerasleeve® | €1.4M | 5 | 50 |
| 74 | Gourmet Delivery by DEKHALU | €1.2M | 5 | 50 |
| 75 | vichemicals | €1.2M | 5 | 50 |
| 76 | Rinstrum Europe GmbH Wägetechnik | €1.2M | 5 | 50 |
| 77 | 3DDruckProfi | €1.1M | 5 | 50 |
| 78 | metallguss eckert gmbh | €1.1M | 5 | 50 |
| 79 | Bernhardt+Partner Architekten | €1.1M | 30 | 50 |
| 80 | Part-time Startup CFO | €1.1M | 5 | 50 |
| 81 | Startup CFO in Teilzeit | €1.1M | 5 | 50 |
| 82 | Fischer Wenzel | €1.0M | 5 | 40 |
| 83 | Pahl Weber-Pahl Architekten | €1.0M | 5 | 40 |
| 84 | GE Fanuc Intelligent Plattforms Europe SA Automatisierungstechnik | €950K | 5 | 40 |
| 85 | Roland Kaupp, Freier Architekt | €925K | 5 | 40 |
| 86 | marksl . planungsgesellschaft | €925K | 5 | 40 |
| 87 | elevat3 | €923K | 5 | 40 |
| 88 | Almoha World GbR | €923K | 5 | 40 |
| 89 | Dieter Frommann Lichtpause1 | €917K | 5 | 40 |
| 90 | Toplink GmbH | €908K | 30 | 40 |
| 91 | teech Education GmbH | €908K | 30 | 40 |
| 92 | Lylu | We are hiring! 💙 | €851K | 5 | 40 |
| 93 | dgg - the 3d pipeline company | €845K | 30 | 40 |
| 94 | intelligent views | €845K | 30 | 40 |
| 95 | mindUp | €834K | 5 | 40 |
| 96 | Junior Comtec – Student consultancy | €813K | 30 | 40 |
| 97 | ICS International AG Identcode Systeme | €813K | 30 | 40 |
| 98 | Tapari Spielwelt | €811K | 5 | 40 |
| 99 | Schlüsseldienst Darmstadt 24h | €755K | 5 | 40 |
| 100 | di-solution gmbh | €644K | 5 | 40 |