| 1 | Diersch & Schröder GmbH & Co. KG | €4.4B | 857 | 69 |
| 2 | OHB SE | €1.2B | 3,292 | 69 |
| 3 | DS card + drive GmbH | €585.0M | -- | 69 |
| 4 | HANSA-FLEX AG | €499.3M | 4,165 | 69 |
| 5 | Enno Roggemann GmbH & Co. KG | €378.4M | 375 | 69 |
| 6 | the bremen university of applied sciences | €343.3M | 3,000 | 70 |
| 7 | Bremen Metall Energie Service | €268.2M | 350 | 70 |
| 8 | Achelis Group | €268.2M | 350 | 70 |
| 9 | Otto Stadtlander GmbH | €251.5M | 26 | 69 |
| 10 | Lerbs GmbH | €229.6M | 350 | 70 |
| 11 | team neusta unternehmensgruppe | €211.5M | 3,000 | 70 |
| 12 | ALCOBRA GmbH | €190.8M | 35 | 69 |
| 13 | Mondelez Deutschland Snacks Production GmbH & Co. KG | €152.6M | 644 | 69 |
| 14 | Bierbaum GmbH & Co. KG | €136.2M | 834 | 69 |
| 15 | G. Theodor Freese GmbH | €115.6M | 350 | 70 |
| 16 | FINKE Mineralölwerk GmbH | €109.7M | 350 | 70 |
| 17 | schütte Herbal Solutions GmbH & Co. KG | €104.1M | 108 | 69 |
| 18 | schütte Home & Living GmbH & Co. KG | €104.1M | 108 | 69 |
| 19 | Allgeier Inovar GmbH | €98.0M | 4 | 69 |
| 20 | NI DE GmbH | €95.8M | 125 | 60 |
| 21 | Bremer Heimstiftung | €91.0M | 2,600 | 69 |
| 22 | Deutsche Gesellschaft zur Rettung Schiffbrüchiger | €87.0M | 321 | 69 |
| 23 | BEGO Bremer Goldschlägerei Wilh. Herbst GmbH & Co. KG | €85.6M | 350 | 60 |
| 24 | Karl Geuther & Co. Holding GmbH & Co. Kommanditgesellschaft | €79.9M | 119 | 69 |
| 25 | Karl Geuther GmbH & Co. KG | €79.9M | 119 | 69 |
| 26 | JW Vermögensverwaltung GmbH | €71.5M | 128 | 69 |
| 27 | OSPIG GmbH | €64.8M | 97 | 69 |
| 28 | Vierzehnte Büttner Schiffahrtsgesellschaft mbH & Co. KG | €53.9M | 2,021 | 69 |
| 29 | Fünfzehnte Büttner Schiffahrtsgesellschaft mbH & Co. KG | €53.9M | 2,021 | 69 |
| 30 | B. Kathmann Bauunternehmung GmbH u. Co. KG | €52.9M | 350 | 60 |
| 31 | ULaaDS - Urban Logistics as an on-Demand Service | €51.0M | 125 | 60 |
| 32 | CoolerU GmbH | €51.0M | 125 | 60 |
| 33 | BLG Cargo Logistics GmbH, Bremen | €51.0M | 125 | 60 |
| 34 | RytleX | €51.0M | 125 | 60 |
| 35 | Karibu Holztechnik GmbH | €49.0M | 125 | 60 |
| 36 | Karibu Holztechnik GmbH | €49.0M | 125 | 60 |
| 37 | EUROGATE GmbH & Co. KGaA, KG | €48.2M | 298 | 69 |
| 38 | SVB-Spezialversand für Yacht- und Bootszubehör GmbH | €46.4M | 40 | 69 |
| 39 | "Neptun" Beteiligungsgesellschaft mbH | €41.6M | 1,488 | 69 |
| 40 | BOCS GmbH | €41.1M | 30 | 60 |
| 41 | IW-NET Project | €41.1M | 30 | 60 |
| 42 | HSB Hochschule Bremen – City University of Applied Sciences | €40.1M | 350 | 60 |
| 43 | Financial Experts Association e.V. (FEA) | €36.4M | 350 | 60 |
| 44 | MARUM - Center for Marine Environmental Sciences, University of Bremen | €36.4M | 350 | 60 |
| 45 | Lantmännen Unibake Germany GmbH & Co. KG | €35.6M | 96 | 69 |
| 46 | CS Parts Logistics GmbH | €34.9M | 2,021 | 69 |
| 47 | Türkische Studentengemeinschaft Bremen - TSB | €33.2M | 350 | 60 |
| 48 | CONTACT Software GmbH | €30.3M | -- | 69 |
| 49 | Tiemann Gruppe | €30.0M | 350 | 60 |
| 50 | Theater Bremen GmbH | €29.7M | -- | 69 |
| 51 | Lloyd Dynamowerke GmbH | €29.2M | 125 | 60 |
| 52 | Lloyd Dynamowerke GmbH | €29.2M | 125 | 60 |
| 53 | cellumation GmbH | €28.2M | 125 | 60 |
| 54 | nwm nordwest-media Servicegesellschaft der Sparkasse Bremen mbH | €26.3M | 125 | 60 |
| 55 | WFB Wirtschaftsförderung Bremen GmbH | €24.7M | 256 | 69 |
| 56 | Berufsbildungswerk Bremen gGmbH | €23.3M | 283 | 69 |
| 57 | Bremedia Produktion GmbH | €23.1M | 350 | 60 |
| 58 | Behälter KG Bremen GmbH & Co | €23.0M | 30 | 60 |
| 59 | siebensonne internationalen handels & beratungs ug | €23.0M | 30 | 60 |
| 60 | Melchers Components | €23.0M | 30 | 60 |
| 61 | GDSC Jacobs University Bremen | €21.3M | 125 | 60 |
| 62 | f.w. neukirch (gmbh & co.) kg | €20.6M | 125 | 60 |
| 63 | ATLANTIC Grand Hotel Bremen | €20.5M | 125 | 60 |
| 64 | BEST WESTERN Hotel Schaper-Siedenburg | €20.5M | 125 | 60 |
| 65 | Johann Erhard Meyer GmbH & Co. KG | €19.7M | 30 | 60 |
| 66 | JACBO Flooring | €18.9M | 125 | 60 |
| 67 | grozema | €18.9M | 125 | 60 |
| 68 | DESKA-IMEX Dr. Schmitz GmbH & Co. KG Import- und Exporthandel | €17.8M | 30 | 60 |
| 69 | Kyocera Senco Deutschland | €17.8M | 30 | 60 |
| 70 | Greensill Bank AG | €16.4M | 78 | 60 |
| 71 | Deosun Schiffahrtsgesellschaft mbH & Co. KG | €16.4M | 3 | 69 |
| 72 | i-tms | €16.1M | 125 | 60 |
| 73 | ROTARY CLUB BREMEN | €15.7M | 125 | 60 |
| 74 | Stevialine GmbH & Co.KG | €15.3M | 30 | 60 |
| 75 | hyBit - Hydrogen for Bremen's industrial transformation | €14.3M | 125 | 60 |
| 76 | Bremer Bereederungsgesellschaft mbH & Co. KG | €13.5M | 6 | 69 |
| 77 | Scanmetals Deutschland GmbH | €13.4M | 2 | 69 |
| 78 | 3d kaufhaus | €12.2M | 30 | 60 |
| 79 | Studio Behnsen | €12.2M | 30 | 60 |
| 80 | profishop | €11.9M | 125 | 60 |
| 81 | die dadego | €11.8M | 30 | 60 |
| 82 | Ingenieurbüro Wichmann GmbH | €11.8M | 30 | 60 |
| 83 | FMC Construction Service1 GmbH | €11.5M | 1 | 69 |
| 84 | fair Finanzpartner OHG | €11.0M | 30 | 60 |
| 85 | willis.com | €11.0M | 30 | 60 |
| 86 | Deomar Schiffahrts-Gesellschaft mbH & Co. KG | €10.9M | 3 | 69 |
| 87 | Sourcetronic Automation GmbH | €10.8M | 30 | 60 |
| 88 | ivbm gmbh | €10.6M | 30 | 60 |
| 89 | Karl Gross Internationale Spedition GmbH | €10.1M | 118 | 69 |
| 90 | GFM Gesellschaft für Micronisierung mbH | €9.6M | 74 | 69 |
| 91 | Ambrian Energy GmbH (formerly HGM Energy) | €9.4M | 30 | 50 |
| 92 | Bremer Mineralölhandel Gesellschaft mit beschränkter Haftung | €8.7M | -- | 69 |
| 93 | Gustav Schramm GmbH | €8.3M | 100 | 69 |
| 94 | Automated Road Transport Forum (ART-Forum) | €8.2M | 30 | 50 |
| 95 | Überseeinsel GmbH | €8.2M | 125 | 50 |
| 96 | bemd e.v. | €8.1M | 5 | 50 |
| 97 | Johanniterhaus Bremen Gemeinnützige GmbH | €7.8M | 141 | 69 |
| 98 | Schünemann GmbH, Georg Filter- u. Armaturenbau | €7.0M | 30 | 50 |
| 99 | nwd nordwest Dienstleistungsgesellschaft der Sparkasse Bremen mbH | €7.0M | 4 | 69 |
| 100 | Leviathan GmbH | €6.8M | 5 | 50 |