| 1 | ARGENX
biotechnology | €2.1B | 419 | 65 | B |
| 2 | Ghent University | €1.8B | 15,500 | 80 | A |
| 3 | Farys
wastewater treatment | €506.3M | 927 | 65 | B |
| 4 | MOLYMET BELGIUM | €359.5M | 155 | 65 | B |
| 5 | ALCO BIO FUEL | €277.0M | 48 | 65 | B |
| 6 | ABLYNX | €122.9M | 406 | 65 | B |
| 7 | UNILIN RESINS
Manufacture of other chemical products n.e.c. | €116.5M | -- | 65 | B |
| 8 | NORTH SEA PORT FLANDERS | €58.8M | 128 | 65 | B |
| 9 | FLI Hospitality Management | €57.5M | 350 | 60 | C+ |
| 10 | ART & CRAFT MEDIA NV | €54.2M | 21 | 65 | B |
| 11 | COMBELL | €51.6M | 128 | 65 | B |
| 12 | ESKO SOFTWARE SPRL | €48.0M | 98 | 65 | B |
| 13 | LAROY GROUP | €47.4M | 98 | 65 | B |
| 14 | COMPAGNIE BELGE DE MANUTENTION | €46.5M | 19 | 65 | B |
| 15 | Concordia n.v. | €45.9M | 125 | 60 | C+ |
| 16 | IFG EXELTO | €43.4M | 227 | 65 | B |
| 17 | POLZER NV | €43.3M | 107 | 65 | B |
| 18 | FUJIREBIO EUROPE | €40.1M | 140 | 65 | B |
| 19 | KONINKLIJKE ATHLETIEK ASSOCIATIE GENT CVBA | €37.5M | 115 | 65 | B |
| 20 | SFRR-E ECR | €36.4M | 350 | 60 | C+ |
| 21 | LEMPAN NV | €35.2M | 10 | 65 | B |
| 22 | 4PS Belgium | €33.1M | 350 | 60 | C+ |
| 23 | IMERYS TALC BELGIUM | €29.0M | 37 | 65 | B |
| 24 | Earthfirst® Biopolymer Films by Sidaplax | €28.6M | 125 | 60 | C+ |
| 25 | DAPHNE SA | €23.8M | 42 | 65 | B |
| 26 | DECOLVENAERE | €19.3M | 9 | 65 | B |
| 27 | SKOG vzw | €19.2M | 350 | 60 | C+ |
| 28 | MG REAL ESTATE | €17.1M | 15 | 65 | B |
| 29 | PSYCHIATRISCH ZIEKENHUIS SINT-CAMILLUS VZW | €16.9M | 202 | 65 | B |
| 30 | Carrier Transicold Belgium
Manufacture of trailers, semi-trailers and caravan | €16.7M | 36 | 65 | B |
| 31 | ALIDES PROPERTIES | €16.6M | 3 | 65 | B |
| 32 | ENFOCUS BVBA | €15.1M | 35 | 65 | B |
| 33 | MaisonRouge | €14.3M | 130 | 60 | C+ |
| 34 | Vlerick Impact Club | €13.0M | 125 | 60 | C+ |
| 35 | BELTMEDIA | €12.2M | 30 | 60 | C+ |
| 36 | B2Build Platform | €11.8M | 30 | 60 | C+ |
| 37 | DOMO CHEMICALS NV | €11.2M | 3 | 65 | B |
| 38 | LOUIS DREYFUS COMPANY BELGIUM
Warehousing and storage | €9.7M | 49 | 65 | B |
| 39 | LEIESTROOM NV | €8.1M | -- | 65 | B |
| 40 | ANNABEL TEXTILES | €7.3M | 41 | 65 | B |
| 41 | FAIR-FRUIT SA/NV | €6.3M | 6 | 65 | B |
| 42 | Jensen Hughes Belgium (former FESG) | €6.0M | 30 | 50 | C |
| 43 | EASTMAN CHEMICAL TECHNOLOGY | €5.8M | 47 | 65 | B |
| 44 | BIM-platform - Het digitale bouwteam | €5.6M | 30 | 50 | C |
| 45 | België Bouwt - Derin BV | €5.6M | 30 | 50 | C |
| 46 | Schelstraete Delacourt Associates | €5.6M | 30 | 50 | C |
| 47 | Trensition - Trendtracker | €5.5M | 30 | 50 | C |
| 48 | journy.io | €5.1M | 30 | 50 | C |
| 49 | HiveCPQ - Product Configurator | €5.1M | 30 | 50 | C |
| 50 | Endare - Your digital innovation partner | €5.1M | 30 | 50 | C |
| 51 | Centr.jobs | €5.1M | 30 | 50 | C |
| 52 | Film Fest Gent - 8 > 19 oktober 2025 | €4.9M | 30 | 50 | C |
| 53 | MEC EUROPE N.V. | €4.8M | 12 | 65 | B |
| 54 | RPO Scientific | €4.3M | 30 | 50 | C |
| 55 | MPCA International BV | €4.3M | 30 | 50 | C |
| 56 | LUMIERE PUBLISHING
Motion picture and video distribution activities | €4.2M | 4 | 65 | B |
| 57 | SDG Conference by BEST | €4.1M | 125 | 50 | C |
| 58 | BUILDING HOTEL MAES | €4.0M | 29 | 65 | B |
| 59 | BLUE LINE STORAGE BV | €3.8M | -- | 65 | B |
| 60 | Dropsolid Digital Experience Company | €3.8M | 125 | 50 | C |
| 61 | Building Physics Group - UGent | €3.4M | 30 | 50 | C |
| 62 | PhairywinD | €3.4M | 30 | 50 | C |
| 63 | Aim for 'Die Sterreweg' | €3.4M | 30 | 50 | C |
| 64 | MaisonRouge - WE ARE HIRING! | €3.3M | 30 | 50 | C |
| 65 | NUCLEUS | €3.2M | 8 | 65 | B |
| 66 | CASA LUKER EUROPE | €3.2M | 1 | 65 | B |
| 67 | UGent i-KNOW | €3.1M | 30 | 50 | C |
| 68 | MOLECUBES NV | €3.1M | 30 | 50 | C |
| 69 | hitch.tools | €3.0M | 5 | 50 | C |
| 70 | vdk bank Gent Damesvolley | €2.9M | 125 | 50 | C |
| 71 | DIGROM ENERGY | €2.8M | -- | 65 | B |
| 72 | Transposia
video game industry | €2.8M | -- | 57 | B |
| 73 | Streamcase NV | €2.7M | 30 | 50 | C |
| 74 | ICONTROLLER | €2.6M | 37 | 65 | B |
| 75 | BALTISSE | €2.6M | 18 | 65 | B |
| 76 | FLANDERS LOGISTICS NV | €2.4M | -- | 65 | B |
| 77 | MARITIME LOGISTICS BORNEM NV | €2.3M | -- | 65 | B |
| 78 | PPE Industrial Distributors Ltd. | €2.2M | 30 | 50 | C |
| 79 | PERRIGO CAPITAL
Wholesale of coffee, tea, cocoa and spices | €2.2M | 22 | 65 | B |
| 80 | NOT YOUR ORDINARY | €2.0M | 5 | 50 | C |
| 81 | shAppit | €2.0M | 5 | 50 | C |
| 82 | Vandekerckhove & Bar | €2.0M | 5 | 50 | C |
| 83 | Ray Care | €2.0M | 5 | 50 | C |
| 84 | ROSE & ZACH | €2.0M | 5 | 50 | C |
| 85 | VIRA | €2.0M | 5 | 50 | C |
| 86 | Engel & Völkers Gent-Centrum | €2.0M | 30 | 50 | C |
| 87 | INTELLIGENT GROUP NV | €1.9M | -- | 65 | B |
| 88 | EASYHOST | €1.8M | 1 | 65 | B |
| 89 | CFS - COMPLETE FENCING SUPPLY BVBA | €1.7M | -- | 65 | B |
| 90 | deJuristen / lesJuristes / theJurists | €1.5M | 30 | 50 | C |
| 91 | Ghent Container Terminal NV | €1.4M | 5 | 50 | C |
| 92 | Ostende Vienne Orient Experience bvba | €1.4M | 5 | 50 | C |
| 93 | BRANDY BVBA | €1.3M | 4 | 65 | B |
| 94 | Smuggler | €1.2M | 5 | 50 | C |
| 95 | EIGEN HEERD IS GOUD WEERD NV | €1.2M | -- | 65 | B |
| 96 | ASTRANOVA (Gent-Belgium) | €1.2M | 5 | 50 | C |
| 97 | hemlin | €1.2M | 5 | 50 | C |
| 98 | HEVADEX BVBA | €1.2M | 11 | 65 | B |
| 99 | MERLIN HOLDCO | €1.1M | -- | 65 | B |
| 100 | Simvesty | €1.1M | 5 | 50 | C |